Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin.
Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin.
复制标题
DOI：
10.1038/s41598-021-95740-x
复制
发表时间：
2021-08-11
影响因子：
4.6
通讯作者：
Weitz DA
中科院分区：
文献类型：
--
作者：
Mohammed D;Park CY;Fredberg JJ;Weitz DA
致瘤细胞的迁移是转移性乳腺癌进展的关键步骤。虽然细胞外基质在乳腺癌细胞迁移中的作用已被广泛描述，但渗透压对肿瘤乳腺群迁移的影响仍不清楚。我们对渗透压对细胞迁移影响的认识大多来自于单细胞水平的研究，没有考虑细胞间的相互作用。在这里，我们研究的影响，适度的渗透压对迁移的细胞团组成的非致瘤性或致瘤性细胞。我们观察到非致瘤细胞的迁移距离和速度下降，但致瘤细胞没有。为了解释这些差异，我们研究了渗透应力如何影响细胞簇的机械性能，并影响它们的体积。我们的研究结果表明，致瘤间充质细胞是不敏感的渗透压比非致瘤细胞，并建议这种差异与较低的表达E-钙粘蛋白。使用EGTA治疗，我们证实，细胞-细胞粘附相互作用的建立是一个关键组成部分的细胞簇的行为，在响应渗透胁迫。这项研究提供了证据间充质致瘤集群的低敏感性，以适度的渗透压，并强调了钙粘蛋白为基础的连接在渗透压响应的重要性。
The migration of tumorigenic cells is a critical step for metastatic breast cancer progression. Although the role of the extracellular matrix in breast cancer cell migration has been extensively described, the effect of osmotic stress on the migration of tumor breast cohorts remains unclear. Most of our understanding on the effect of osmotic stresses on cell migration comes from studies at the level of the single cell in isolation and does not take cell–cell interactions into account. Here, we study the impact of moderate osmotic stress on the migration of cell clusters composed of either non-tumorigenic or tumorigenic cells. We observe a decrease in migration distance and speed for non-tumorigenic cells but not for tumorigenic ones. To explain these differences, we investigate how osmotic stress impacts the mechanical properties of cell clusters and affects their volumes. Our findings show that tumorigenic mesenchymal cells are less sensitive to osmotic stress than non-tumorigenic cells and suggest that this difference is associated with a lower expression of E-cadherin. Using EGTA treatments, we confirm that the establishment of cell–cell adhesive interactions is a key component of the behavior of cell clusters in response to osmotic stress. This study provides evidence on the low sensitivity of mesenchymal tumorigenic clusters to moderate osmotic stress and highlights the importance of cadherin-based junctions in the response to osmotic stress.
登录
查看更多内容
E-钙粘蛋白缺陷型细胞在质膜组织，动力学和功能中具有合成的致命性脆弱性。
DOI：
10.1007/s10120-018-0859-1
发表时间：
2019-03
期刊：
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
影响因子：
--
作者：
Godwin TD;Kelly ST;Brew TP;Bougen-Zhukov NM;Single AB;Chen A;Stylianou CE;Harris LD;Currie SK;Telford BJ;Beetham HG;Evans GB;Black MA;Guilford PJ
通讯作者：
Guilford PJ
N-钙粘着蛋白在乳腺癌细胞中的外源表达会诱导细胞迁移，侵袭和转移。
影响因子：
7.8
作者：
Hazan, R B;Phillips, G R;Qiao, R F;Norton, L;Aaronson, S A
通讯作者：
Aaronson, S A
影响因子：
3.7
作者：
Cheng G;Tse J;Jain RK;Munn LL
通讯作者：
Munn LL
影响因子：
12.4
作者：
Criollo, A.;Maiuri, M. C.;Kroemer, G.
通讯作者：
Kroemer, G.
DOI：
10.1002/advs.202002643
发表时间：
2020-11
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Dumitru AC;Mohammed D;Maja M;Yang J;Verstraeten S;Del Campo A;Mingeot-Leclercq MP;Tyteca D;Alsteens D
通讯作者：
Alsteens D