1,2,3,4,6-Penta-O-galloyl-beta-D-glucose protects rat neuronal cells (Neuro 2A) from hydrogen peroxide-mediated cell death via the induction of heme oxygenase-1
1,2,3,4,6-Penta-O-galloyl-beta-D-glucose protects rat neuronal cells (Neuro 2A) from hydrogen peroxide-mediated cell death via the induction of heme oxygenase-1
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DOI：
10.1016/s0304-3940(02)00513-x
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发表时间：
2002-08-09
影响因子：
2.5
通讯作者：
Chung, HT
中科院分区：
文献类型：
--
作者：
Choi, BM;Kim, HJ;Chung, HT
牡丹是一种重要的中药材，在许多传统方剂中都有使用。1，2，3，4，6-五-O-没食子酰基-β-D-葡萄糖(PGG)是该药材的主要成分，具有较强的抗氧化、抗突变和抗增殖作用。在本研究中，我们研究了PGG对神经元血红素加氧酶-1(HO-1)表达的影响，HO-1是一种可将血红素降解为神经活性分子的应激蛋白，一氧化碳和抗氧化剂胆绿素。PGG(10-50微米)对Neuro 2A细胞HO-1mRNA、蛋白表达和血红素加氧酶活性的影响呈浓度和时间依赖性。有趣的是，用PGG对神经细胞进行预处理后，细胞对过氧化氢的抵抗力增强。这种细胞保护作用可被血红素加氧酶抑制剂锌原卟啉IX逆转。本研究表明，PGG可通过诱导HO-1基因表达来保护神经细胞免受氧化应激的损伤。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
The root of Paeonia suffruticosa ANDREWS is an important Chinese crude drug used in many traditional prescriptions. 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose (PGG), a major component of this crude drug, has been shown to possess potent anti-oxidant, anti-mutagenic and anti-proliferative effects. In the present study, we examined the effect of PGG on the expression of neuronal heme oxygenase-1 (HO-1), an inducible stress protein that degrades heme to the neuroactive molecule, carbon monoxide and the anti-oxidant, biliverdin. Exposure of Neuro 2A cells to PGG (10-50 muM) resulted in a concentration- and time-dependent induction of HO-1 mRNA, and protein expressions and heme oxygenase activity. Interestingly, pretreatment of the neuronal cells with PGG resulted in enhanced cellular resistance to hydrogen peroxide. This cytoprotective effect was reversed by zinc protoporphyrin IX, an inhibitor of heme oxygenase. This study showed that PGG could protect neuronal cells from oxidative stress via the induction of HO-1 gene expression. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.