Highly sensitive surface plasmon resonance biosensor for the detection of HIV-related DNA based on dynamic and structural DNA nanodevices
Highly sensitive surface plasmon resonance biosensor for the detection of HIV-related DNA based on dynamic and structural DNA nanodevices
复制标题
基于动态和结构DNA纳米器件的用于检测HIV相关DNA的高灵敏表面等离子体共振生物传感器
DOI：
10.1016/j.bios.2017.08.042
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发表时间：
2018-02-15
影响因子：
12.6
通讯作者：
Yan, Yurong
中科院分区：
文献类型：
--
作者：
Diao, Wei;Tang, Min;Yan, Yurong
早期发现、诊断和治疗人类免疫缺陷病毒（HIV）感染是降低艾滋病（AIDS）死亡率的关键。在我们的研究中，一种创新的表面等离子体共振（SPR）生物传感策略已被开发用于基于熵驱动的链置换反应（ESDRs）和双层DNA四面体（DDTs）的HIV相关DNA的高灵敏度检测。ESDRs作为一种无酶无标记的信号放大回路，可以被靶DNA特异性地触发，导致靶DNA的循环利用，形成丰富的双链DNA（dsDNA）产物。随后，dsDNA产物与固定的发夹捕获探针结合，并进一步与DDTs纳米结构联合收割机结合。由于ESDRs的高效率和DDTs的大分子量，SPR响应信号显着增强。该SPR生物传感器可在1 pM ~ 150 nM的线性范围内灵敏、特异地检测目标DNA，检测限为48 fM。整个检测过程可在60 min内完成，准确度高，重复性好。特别是，所开发的SPR生物传感器被成功地用于分析复杂生物样品中的目标DNA，表明所开发的策略是有希望的快速和早期的临床诊断HIV感染。
Early detection, diagnosis and treatment of human immune deficiency virus (HIV) infection is the key to reduce acquired immunodeficiency syndrome (AIDS) mortality. In our research, an innovative surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive detection of HIV-related DNA based on entropy-driven strand displacement reactions (ESDRs) and double-layer DNA tetrahedrons (DDTs). ESDRs as enzyme-free and label-free signal amplification circuit can be specifically triggered by target DNA, leading to the cyclic utilization of target DNA and the formation of plentiful double-stranded DNA (dsDNA) products. Subsequently, the dsDNA products bind to the immobilized hairpin capture probes and further combine with DDTs nanostructures. Due to the high efficiency of ESDRs and large molecular weight of DDTs, the SPR response signal was enhanced dramatically. The proposed SPR biosensor could detect target DNA sensitively and specifically in a linear range from 1 pM to 150 nM with a detection limit of 48 fM. In addition, the whole detecting process can be accomplished in 60 min with high accuracy and duplicability. In particular, the developed SPR biosensor was successfully used to analyze target DNA in complex biological sample, indicating that the developed strategy is promising for rapid and early clinical diagnosis of HIV infection.