Pyrokinin/PBAN-like peptides in the central nervous system of Drosophila melanogaster
Pyrokinin/PBAN-like peptides in the central nervous system of Drosophila melanogaster
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DOI：
10.1007/s00441-001-0467-x
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发表时间：
2001-12-01
影响因子：
3.6
通讯作者：
Jurenka, RA
中科院分区：
文献类型：
--
作者：
Choi, MY;Rafaeli, A;Jurenka, RA
在昆虫中发现的焦激肽/信息素生物合成激活神经肽（PBAN）家族的肽的特征在于5个氨基酸的C-末端序列FXPRLamide。五肽是多种生理功能所需的活性核心，包括刺激雌蛾信息素的生物合成，刺激肌肉收缩，诱导家蚕胚胎滞育，以及刺激一些幼虫的黑化。最近，这个家族的肽已被牵连在加速形成的蛹在双翅目。利用生物测定和免疫细胞化学技术，我们证明存在的pyrokinin/PBAN样肽在果蝇中枢神经系统。用离体的棉铃虫幼虫神经系统和成虫头、体，研究了棉铃虫和谷棉铃虫的促信息素活性。黑腹菌针对PBAN的C-末端的多克隆抗血清揭示了幼虫和成年苍蝇的中枢神经系统中的细胞体和轴突的位置。免疫反应物质中检测到至少三组的神经元在食管下神经节的3龄幼虫，蛹，和成人。幼虫和成虫的环腺均含有免疫反应性。成人脑-食管下神经节复合体具有额外的神经元。幼虫和成虫融合的腹侧神经节包含三对神经元，它们将轴突发送到与腹部神经系统相连的神经血管器官。这些结果表明，D.黑胃神经系统含有pyrokinin/PBAN样肽，并且这些肽可以释放到血淋巴中。
The pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides found in insects is characterized by a 5-amino-acid C-terminal sequence, FXPRLamide. The pentapeptide is the active core required for diverse physiological functions, including stimulation of pheromone biosynthesis in female moths, stimulation of muscle contraction, induction of embryonic diapause in Bombyx mori, and stimulation of melanization in some larval moths. Recently, this family of peptides has been implicated in accelerating the formation of the puparium in a dipteran. Using bioassay and immunocytochemical techniques, we demonstrate the presence of pyrokinin/PBAN-like peptides in the central nervous system of Drosophila melanogaster. Pheromonotropic activity was shown in the moths Helicoverpa zea and Helicoverpa armigera by using dissected larval nervous systems and adult heads and bodies of D. melanogaster. Polyclonal antisera against the C-terminal ending of PBAN revealed the location of cell bodies and axons in the central nervous systems of larval and adult flies. Immunoreactive material was detected in at least three groups of neurons in the subesophageal ganglion of 3rd instar larvae, pupae, and adults. The ring gland of both larvae and adults contained immunoreactivity. Adult brain-subesophageal ganglion complex possessed additional neurons. The fused ventral ganglia of both larvae and adults contained three pairs of neurons that sent their axons to a neurohemal organ connected to the abdominal nervous system. These results indicate that the D. melanogaster nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph.