Subgingival microbial profiles in refractory periodontal disease
Subgingival microbial profiles in refractory periodontal disease
复制标题
DOI：
10.1034/j.1600-051x.2002.290313.x
复制
发表时间：
2002-03-01
影响因子：
6.7
通讯作者：
Haffajee, AD
中科院分区：
文献类型：
--
作者：
Socransky, SS;Smith, C;Haffajee, AD
背景/目的：本研究的目的是检测与难治性牙周炎相关的龈下微生物谱，并在牙周健康、牙周保养良好的老年牙周炎患者和未经治疗的牙周炎患者中寻找相关的微生物谱。方法：36例受试者被定义为难治性牙周炎患者，根据根面刮除和根面平整、手术和全身应用抗生素后进一步附着丧失的情况。在基线时，从每个受试者的每颗牙齿的近中面取890个菌斑样本(平均值/受试者=24.7)，并用棋盘DNA-DNA杂交法分别处理它们的40个龈下分类群的含量。使用和弦系数和平均未加权连锁排序对受试者物种计数内的平均值进行聚类分析。利用Kruskal Wallis检验，发现个体和物种复合体的聚类群之间存在显著差异。将难治性牙周炎患者与27例牙周健康患者(n菌斑样本=708例)、35例牙周状况良好的老年人(n菌斑样本=801例)和115例未经治疗的成人牙周炎患者(n菌斑样本=2871例)的菌谱进行比较。结果：36例难治性牙周炎患者中，28例分为4类，相似性为29%。40个物种中的10个和7个复合体中的4个在集群之间存在显著差异。剖面(簇)I(n=4)以高比例的“黄”和“绿”复合种为特征，剖面II(n=3)以低总数和高比例的“橙”和“紫”复合种为特征，剖面III(n=9)以放线菌和“紫色”复合种的高总数和高计数为特征，剖面IV(n=12)以高比例的“红”和“橙”复合种为特征。用来自27名牙周健康、35名经过良好维护的老年人和115名未治疗的成人牙周炎受试者的4380(平均24.7)个基线菌斑样本的微生物数据对每组的平均轮廓进行聚类分析。形成12个类群，相似性为41%。在3个聚类组中检测到3个难治性轮廓。配置文件II包括1名健康牙周炎患者、1名老年患者和4名未经治疗的牙周炎患者；配置文件III包括1名健康牙周炎患者、2名老年人和12名牙周炎患者；配置文件IV包括1名健康牙周炎患者和5名未经治疗的牙周炎患者。在非难治性受试者中未检测到的图谱以链球菌为主。9个星团不含难熔剖面。11.1%的健康受试者、8.6%的老年受试者和18.3%的牙周炎受试者聚集在表现出难治菌群的群体中。在尚未表现出顽固性疾病的受试者中可以检测到“耐受性微生物图谱”。
Background/aim: The purpose of the present investigation was to examine subgingival microbial profiles associated with refractory periodontitis and to seek such profiles in periodontally healthy, periodontally well-maintained elder and untreated periodontitis subjects.Methods: 36 subjects were defined as refractory on the basis of further attachment loss after scaling and root planing, surgery and systemically administered antibiotics. A total of 890 subgingival plaque samples (mean/subject=24.7) were taken from the mesial aspect of each tooth in each subject at baseline and individually processed for their content of 40 subgingival taxa using checker-board DNA-DNA hybridization. Cluster analysis was performed on mean within subject species counts using the chord coefficient and an average unweighted linkage sort. Significant differences among clusters for individual and complexes of species were sought using the Kruskal Wallis test. The microbial profiles of the refractory subjects were compared with those of 27 periodontally healthy subjects (n plaque samples=708), 35 periodontally well-maintained elder subjects (n plaque samples=801) and 115 untreated adult periodontitis subjects (n plaque samples=2871).Results: 28 of 36 refractory subjects fell into 4 clusters with >29% similarity. 10 of 40 species and 4 of 7 complexes differed significantly among clusters. Profile (Cluster) I (n=4) was characterized by high proportions of "yellow" and "green" complex species, profile II (n=3) by low total counts and high proportions of "orange" and "purple" complex species, profile III (n=9) by high total counts and counts of Actinomyces and "purple" complex species, profile IV (n=12) by high proportions of "red" and "orange" complex species. The mean profiles of each cluster were subjected to cluster analysis with microbial data from 4380 (mean 24.7) baseline subgingival plaque samples from 27 periodontally healthy, 35 treated, well-maintained elders and 115 untreated adult periodontitis subjects. 12 clusters were formed with >41% similarity. 3 of the refractory profiles were detected in 3 cluster groups. Profile II in a cluster of 1 healthy, 1 elder and 4 untreated periodontitis subjects; profile III in a cluster of 1 healthy, 2 elder and 12 periodontitis subjects; Profile IV, with 1 healthy and 5 untreated periodontitis subjects. The profile not detected in non refractory subjects was dominated by Streptococcus species. 9 clusters did not harbor refractory profiles. 11.1% of healthy, 8.6% of elder and 18.3% of periodontitis subjects were in clusters exhibiting refractory microbial profiles.Conclusions: 4 subgingival microbial profiles were detected among refractory subjects. "Refractory microbial profiles" could be detected in subjects who had not yet exhibited refractory disease.