IMMUNOGENETIC ANALYSIS OF H-2 MUTATIONS .6. CROSS-REACTIVITY IN CELL-MEDIATED LYMPHOLYSIS BETWEEN TNP-MODIFIED CELLS FROM H-2 MUTANT STRAINS
IMMUNOGENETIC ANALYSIS OF H-2 MUTATIONS .6. CROSS-REACTIVITY IN CELL-MEDIATED LYMPHOLYSIS BETWEEN TNP-MODIFIED CELLS FROM H-2 MUTANT STRAINS
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DOI：
10.1007/bf01575657
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发表时间：
1977-01-01
期刊：
影响因子：
3.2
通讯作者：
KLEIN, J
中科院分区：
文献类型：
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作者：
FORMAN, J;KLEIN, J
携带H-2Kb [组织相容性]等位基因并在体外对TNP[三硝基苯基]修饰的刺激细胞致敏的脾细胞，对携带H-2Kb等位基因突变（单倍型H-2ba、H-2bd和H-2bf）的TNP修饰的靶细胞显示出细胞毒性作用。在TNP-CML [细胞介导的淋巴细胞溶解]中，在相反方向观察到类似的交叉反应性。携带H-2Kk等位基因并对TNP修饰的刺激物致敏的脾细胞对携带H-2Kk等位基因突变（单倍型H-2ka）的TNP修饰的靶细胞显示出细胞毒性作用，反之亦然。这些试验中的效应细胞在补体存在下对抗T [胸腺衍生]细胞血清敏感，免疫培养物的上清液不会诱导非免疫细胞显示细胞毒性作用。从突变体和野生型菌株的H-2b单倍型的效应细胞的滴定表明没有检测到的定量差异，其活动。TNP-CML的交叉反应性显然发生在最近经历了H-2复合物突变的密切相关的同种异体菌株中。
Spleen cells, carrying the H-2Kb [histocompatibility] allele and sensitized against TNP[trinitrophenyl]-modified stimulator cells in vitro, displayed a cytotoxic effect against TNP-modified target cells carrying a mutation in the H-2Kb allele (haplotypes H-2ba, H-2bd and H-2bf). Similar crossreactivity in TNP-CML [cell mediated lympholysis] was observed in the reciprocal direction. Spleen cells carrying the H-2Kk allele and sensitized against TNP-modified stimulators displayed a cytotoxic effect against TNP-modified target cells carrying a mutation in the H-2Kk allele (haplotype H-2ka) and vice versa. Effector cells in these assays were sensitive to anti-T [thymus-derived] cell serum in the presence of complement, and supernatants from immune cultures did not induce nonimmune cells to display a cytotoxic effect. Titration of effector cells from mutant and wild-type strains of the H-2b haplotype indicated no detectable quantitative differences in their activities. Crossreactivity in TNP-CML apparently occurs in closely related allogeneic strains that have recently undergone mutation in the H-2 complex.