EXPRESSION OF WHEY ACIDIC PROTEIN (WAP) GENES IN TISSUES OTHER THAN THE MAMMARY-GLAND IN NORMAL AND TRANSGENIC MICE EXPRESSING MWAP/HGH FUSION GENE
EXPRESSION OF WHEY ACIDIC PROTEIN (WAP) GENES IN TISSUES OTHER THAN THE MAMMARY-GLAND IN NORMAL AND TRANSGENIC MICE EXPRESSING MWAP/HGH FUSION GENE
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DOI：
10.1002/mrd.1080410402
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发表时间：
1995-08-01
影响因子：
2.5
通讯作者：
TACHI, C
中科院分区：
文献类型：
--
作者：
WEN, JY;KAWAMATA, Y;TACHI, C
乳清酸性蛋白（WAP）是啮齿动物乳汁中分泌的一种主要乳清蛋白。小鼠WAP （mWAP）基因一直被认为只在乳腺中表达。然而，一些与mWAP启动子融合并作为转基因人工导入动物基因组的异源基因不仅在乳腺中表达，而且在其他组织中也表达。本研究采用反转录聚合酶链反应（RT-PCR）技术，研究了内源性WAP基因在正常小鼠和携带hGH基因偶联mWAP启动子序列的转基因小鼠组织中的表达规律。结果显示，由mWAP启动子驱动的基因，无论是内源基因还是转基因基因，在哺乳期正常雌性小鼠乳腺以外的多种组织中都有转录，尽管表达水平普遍较低。和乳腺一样，WAP基因在大脑和肝脏中的表达也受到生殖阶段的调节。然而，内源性WAP基因在成熟处女转基因雌性小鼠中的组织表达分布与哺乳期正常雌性小鼠相同。(C) 1995 Wiley-Liss, Inc。
Whey acidic protein (WAP) is a major whey protein secreted in rodents' milk. Murine WAP (mWAP) genes have been assumed to be expressed solely in the mammary gland. However, several heterologous genes fused with the mWAP promoter and artificially introduced into animal genomes as transgene were expressed not only in the mammary gland but also in other tissues as well. In the present study, we investigated, by means of the reverse transcription polymerase chain reaction (RT-PCR), the patterns of expression of endogenous WAP genes in tissues of normal mice and in transgenic mice carrying hGH gene coupled to the mWAP promoter sequence. The results revealed that the genes driven by the mWAP promoter, regardless of whether they are endogenous genes or transgenes, were transcribed in a variety of tissues other than the mammary gland of lactating normal female mice, although the expression levels are generally low. The expression of WAP genes in the cerebrum and the liver is regulated, as in the mammary gland, according to the reproductive stages. However, the tissue distribution of endogenous WAP gene expression in mature virgin transgenic female mice was the same as that in lactating normal female mice. (C) 1995 Wiley-Liss, Inc.