RGD peptides grafting onto poly(ethylene terephthalate) with well controlled densities
RGD peptides grafting onto poly(ethylene terephthalate) with well controlled densities
复制标题
DOI：
10.1016/j.bioeng.2007.07.012
复制
发表时间：
2007-11-01
期刊：
影响因子：
--
通讯作者：
Durrieu, M. C.
中科院分区：
文献类型：
--
作者：
Chollet, C.;Chanseau, C.;Durrieu, M. C.
本研究的目的是接枝RGD肽与良好控制的密度到聚（对苯二甲酸乙二醇酯）（PET）膜表面。仿生改性通过四步反应程序进行：表面改性以在聚合物表面上产生-COOH基团，偶联剂接枝和最后固定化肽。这项工作的独创性是评估几个接枝密度肽。甲苯胺蓝和高分辨率μ成像仪（使用[H-3]-Lys）用于评价密度。此外，mu-成像仪显示出在苛刻条件下处理时接枝到表面上的肽的稳定性。所提出的方法的益处与接枝到表面上的肽的不同浓度以及RGD肽与整联蛋白受体相互作用的能力有关。(C)2007 Elsevier B. V.保留所有权利。
The aim of this study was to graft RGD peptides with well controlled densities onto poly(ethylene terephthalate) (PET) film surfaces. Biomimetic modifications were performed by means of a four-step reaction procedure: surface modification in order to create -COOH groups onto polymer surface, coupling agent grafting and finally immobilization of peptides. The originality of this work is to evaluate several grafted densities peptides. Toluidine blue and high-resolution mu-imager (using [H-3]-Lys) were used to evaluate densities. Moreover, mu-imager has exhibited the stability of peptides grafted onto the surface when treated under harsh conditions. Benefits of the as-proposed method were related to the different concentrations of peptides grafted onto the surface as well as the capacity of RGD peptide to interact with integrin receptors. (C) 2007 Elsevier B.V. All rights reserved.