Quantitative Assessment of Steroid Hormone Binding Sites by Thaw-Mount Autoradiography 1 , 2
Quantitative Assessment of Steroid Hormone Binding Sites by Thaw-Mount Autoradiography 1 , 2
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通过解冻放射自显影对类固醇激素结合位点进行定量评估 1 , 2
DOI：
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发表时间：
1981
影响因子：
3.2
通讯作者：
P. Tuohimaa
中科院分区：
文献类型：
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作者：
Walter E. Stumpf;Madhabananda Sar;T. J. Zuber;E. Soini;P. Tuohimaa
介绍了一种对单个细胞中类固醇激素和其他物质的核受体进行定量评估的方法。采用的是解冻挂载放射自显影，这是我们实验室早期开发的程序。在豚鼠子宫组织中测得的银粒产额(比活度)为每银粒16.6次崩解。本文提出了测定样品组织中~3H-雌二醇、~3H-己烯雌酚和~3H-醛固酮的综合公式，并进行了应用。与文献中基于全子宫匀浆和生化分析的数据进行比较，如果将后者合并，则显示与放射自显影数据类似的值。汇集的数据显示，每个子宫核有12-14,000个~3H-雌二醇分子，而靶细胞亚群在每个核5,000到28,000个分子之间变化。
A procedure for the quantitative assessment of nuclear receptors for steroid hormones—and other substances—in individual cells is presented. Thaw-mount autoradiography, a procedure developed earlier in our laboratory, is utilized. The silver grain yield (specific activity) is 16.6 disintegrations per silver grain as determined for tritium in guinea pig uterine tissues. An integrated formula is presented and applied for 3H-estradioI, 3H-diethyl-stilbestrol, and 3H-aldosterone in sampled tissues. A comparison with data derived from the literature that are based on the homogenization of whole uteri and biochemical analysis shows comparable values with the autoradiographic data if the latter are pooled. The pooled data indicate 12–14,000 molecules of 3H-estradiol per uterine nucleus, while subpopulations of target cells vary between 5,000 and 28,000 per nucleus.