The genome of woodland strawberry (Fragaria vesca).
The genome of woodland strawberry (Fragaria vesca).
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野草莓（Fragaria vesca，2n = 2x = 14）是一种多功能的实验植物系统。这种小型多年生草本植物具有小的基因组（240 Mb），易于遗传转化，并且与栽培草莓（Fragaria × ananassa）和其他经济上重要的蔷薇科植物具有实质性的序列同一性。在这里，我们报告的草案F。vesca基因组，使用第二代技术测序到×39覆盖率，从头组装，然后锚定到遗传连锁图谱上，形成7个假染色体。这个二倍体草莓序列缺乏在其他蔷薇科植物中看到的大的基因组重复。基因预测模型确定了34，809个基因，其中大部分得到了转录组图谱的支持。鉴定了对有价值的园艺性状包括风味、营养价值和开花时间至关重要的基因。草莓属和李属之间的大共线关系预测了一个假设的祖先蔷薇科基因组，有9条染色体。新的系统发育分析的154个蛋白质编码基因表明，分配杨树锦葵科，而不是Fabidae，是有道理的。
The woodland strawberry, Fragaria vesca (2n = 2x = 14), is a versatile experimental plant system. This diminutive herbaceous perennial has a small genome (240 Mb), is amenable to genetic transformation and shares substantial sequence identity with the cultivated strawberry (Fragaria × ananassa) and other economically important rosaceous plants. Here we report the draft F. vesca genome, which was sequenced to ×39 coverage using second-generation technology, assembled de novo and then anchored to the genetic linkage map into seven pseudochromosomes. This diploid strawberry sequence lacks the large genome duplications seen in other rosids. Gene prediction modeling identified 34,809 genes, with most being supported by transcriptome mapping. Genes critical to valuable horticultural traits including flavor, nutritional value and flowering time were identified. Macrosyntenic relationships between Fragaria and Prunus predict a hypothetical ancestral Rosaceae genome that had nine chromosomes. New phylogenetic analysis of 154 protein-coding genes suggests that assignment of Populus to Malvidae, rather than Fabidae, is warranted.