Agricultural dust derived bacterial extracellular vesicle mediated inflammation is attenuated by DHA.
Agricultural dust derived bacterial extracellular vesicle mediated inflammation is attenuated by DHA.
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DOI：
10.1038/s41598-023-29781-9
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发表时间：
2023-02-16
影响因子：
4.6
通讯作者：
Romberger, Debra J.
中科院分区：
文献类型：
--
作者：
Heires, Art J.;Samuelson, Derrick;Villageliu, Daniel;Nordgren, Tara M.;Romberger, Debra J.
饮食中的长链omega-3多不饱和脂肪酸(n-3 PUFA)及其亲解代谢物对动脉粥样硬化性疾病具有保护作用，并可改善系统性炎症状况，包括红斑狼疮、牛皮癣和支气管哮喘。有机生物气雾剂吸入是一种常见的有害危险，与农业职业有关，如在猪集中动物饲养作业(CAFO)中工作，已知会增加患哮喘和慢性阻塞性肺病等呼吸系统疾病的风险。几乎所有的细胞都分泌膜结合的囊泡(细胞外囊泡，EVS)，这些囊泡具有在细胞之间传递蛋白质、核酸和脂质信号媒介的能力。使用聚合物为基础的分离技术(ExoQuick，PEG)，然后超速离心，从CAFO粉尘提取物中分离EV，并对其进行定量和部分表征。在这里，我们研究了n-3PUFA二十二碳六烯酸(DHA)作为n-6到n-3 PUFA混合物的一个组成部分在解决体外暴露于农业有机粉尘携带的EVS引起的炎性损伤中的作用。原代人支气管上皮细胞、成纤维细胞和单核细胞来源的巨噬细胞暴露于从猪CAFO粉尘中分离的EVS。在EV诱导的损伤恢复过程中，用n-6和n-3多不饱和脂肪酸混合物处理细胞。CAFO粉尘提取物(DE)被发现含有对暴露于完全DE的总体后果有显著贡献的EVS。富含DHA的PUFA比率呈剂量依赖性抑制DE来源的EV诱导的促炎细胞因子的释放。富含DHA的多不饱和脂肪酸比例还逆转了EVS对肺基质支架再细胞化的破坏作用，加速了伤口愈合，并刺激了促分解介质的释放。这些结果强调了在体外细胞反应的背景下，n-3多不饱和脂肪酸作为抗炎化合物在从携带EV的环境粉尘暴露中恢复的重要性，这证明了未来的翻译研究。
Dietary long-chain omega-3 polyunsaturated fatty acids (n-3 PUFA) and their pro-resolving metabolites are protective against atherosclerotic disease, and ameliorate systemic inflammatory conditions including lupus erythematosus, psoriasis, and bronchial asthma. Organic bioaerosol inhalation is a common and injurious hazard associated with agricultural occupations such as work in swine concentrated animal feeding operations (CAFOs) and is known to increase the risk for developing respiratory conditions such as asthma and COPD. Nearly all cells secrete membrane-bound vesicles (extracellular vesicles, EVs) that have the capacity to transmit protein, nucleic acid, and lipid signaling mediators between cells. Using a polymer-based isolation technique (ExoQuick, PEG) followed by ultracentrifugation, EVs were isolated from CAFO dust extracts, and were quantified and partially characterized. Here, we investigated the role of the n-3 PUFA docosahexaenoic acid (DHA) as a component of n-6 to n-3 PUFA mixtures used to recapitulate physiologically relevant dietary ratios in the resolution of inflammatory injury caused by exposure to EVs carried by agricultural organic dust in vitro. Primary human bronchial epithelial cells, fibroblasts and monocyte-derived macrophages were exposed to EVs isolated from swine CAFO dust. Cells were treated with mixtures of n-6 and n-3 PUFA during recovery from the EV-induced injury. CAFO dust extract (DE) was found to contain EVs that contributed significantly to the overall consequences of exposure to complete DE. DHA-rich PUFA ratios inhibited DE-derived EV-induced proinflammatory cytokine release dose-dependently. DHA-rich PUFA ratios also reversed the damaging effects of EVs on recellularization of lung matrix scaffolds, accelerated wound healing, and stimulated the release of pro-resolution mediators. These results underscore the importance of n-3 PUFA as anti-inflammatory compounds during recovery from EV-laden environmental dust exposure in the context of cellular responses in vitro, warranting future translational studies.
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影响因子：
7.7
作者：
Beghini F;McIver LJ;Blanco-Míguez A;Dubois L;Asnicar F;Maharjan S;Mailyan A;Manghi P;Scholz M;Thomas AM;Valles-Colomer M;Weingart G;Zhang Y;Zolfo M;Huttenhower C;Franzosa EA;Segata N
通讯作者：
Segata N
DOI：
10.1152/ajplung.90576.2008
发表时间：
2010-02-01
影响因子：
4.9
作者：
Fukumoto, Jutaro;Harada, Chika;Nakanishi, Yoichi
通讯作者：
Nakanishi, Yoichi
DOI：
10.20517/evcna.2020.09
发表时间：
2021
期刊：
Extracellular vesicles and circulating nucleic acids
影响因子：
--
作者：
Comfort N;Bloomquist TR;Shephard AP;Petty CR;Cunningham A;Hauptman M;Phipatanakul W;Baccarelli A
通讯作者：
Baccarelli A
DOI：
10.1016/0300-9629(92)90551-z
发表时间：
1992-02-01
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY
影响因子：
--
作者：
CHARNOCK, JS;ABEYWARDENA, MY;MCLENNAN, PL
通讯作者：
MCLENNAN, PL
DOI：
10.1186/cc8844
发表时间：
2010
期刊：
Critical care (London, England)
影响因子：
--
作者：
Barbosa VM;Miles EA;Calhau C;Lafuente E;Calder PC
通讯作者：
Calder PC