CELL LINEAGE OF ZEBRAFISH BLASTOMERES .2. FORMATION OF THE YOLK SYNCYTIAL LAYER
CELL LINEAGE OF ZEBRAFISH BLASTOMERES .2. FORMATION OF THE YOLK SYNCYTIAL LAYER
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DOI：
10.1016/0012-1606(85)90011-9
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
LAW, RD
中科院分区：
文献类型：
--
作者：
KIMMEL, CB;LAW, RD
研究了斑马鱼卵黄合胞层（YSL）形成的卵裂球的染料偶联性和细胞系。YSL是一个多核的非卵黄细胞质层，位于巨大卵黄细胞的1极的囊胚下面。卵黄在卵黄细胞第10次（有时是第9次）卵裂时，由一组卵裂球（称为边缘卵裂球）向卵黄细胞分裂而形成。在YSL形成之前，卵裂球边缘具有与卵黄细胞的细胞质桥，向细胞中注射荧光素-葡聚糖发现，卵黄细胞与胚胚之间的桥在此之后就不存在了。路西法黄的注射显示，在YSL形成后不久，卵黄细胞和囊胚被染料偶联，可能是通过间隙连接，这种偶联在早期原肠胚形成过程中逐渐消失。谱系分析表明，并非所有早期边缘卵裂球的后代都参与YSL的形成。尽管一些边缘卵裂球的后代在连续的卵裂过程中仍然留在边缘，但无论是隔室还是严格的谱系模型都不足以解释YSL的起源。细胞在胚层边缘的位置，而不是细胞的谱系，显然决定了YSL细胞的命运。
Dye coupling and cell lineages of blastomeres that participate in the formation of the yolk syncytial layer (YSL) in the zebrafish Brachydanio rerio were examined. The YSL is a multinucleate layer of nonyolky cytoplasm underlying the cellular blastoderm at 1 pole of the giant yolk cell. It forms at the time of the 10th (sometimes 9th) cleavage by a collapse of a set of blastomeres, termed marginal blastomeres, into the yolk cell. Marginal blastomeres possess cytoplasmic bridges to the yolk cell before the YSL forms and injections of fluorescein-dextran into the cells revealed that bridges between the yolk cell and blastoderm do not persist after this time. Injections of Lucifer yellow revealed that shortly after the YSL forms the yolk cell and blastoderm are dye coupled, presumably by gap junctions, and that this coupling disappears gradually during early gastrulation. Lineage analyses revealed that not all of the progeny of early marginal blastomeres participate in YSL formation. Although some descendants of marginal blastomeres remained on the margin during successive cleavages, neither compartment nor strict lineage models are sufficient to explain the origin of the YSL. The cell position on the blastoderm margin, and not the cell''s lineage, apparently determines YSL cell fate.