Characterization of lactic acid bacteria-based probiotics as potential heavy metal sorbents
Characterization of lactic acid bacteria-based probiotics as potential heavy metal sorbents
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DOI：
10.1111/j.1365-2672.2012.05284.x
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发表时间：
2012-06-01
影响因子：
4
通讯作者：
Wei, M. Q.
中科院分区：
文献类型：
--
作者：
Bhakta, J. N.;Ohnishi, K.;Wei, M. Q.
目的：对乳酸菌(LAB)进行分离鉴定，确定其作为重金属(镉、铅)吸附益生菌的潜力。方法和结果：本研究使用53个环境样品(泥浆和污泥)，采用备用平板技术分离耐镉、抗铅实验室。从这些样本中共分离出255株抗镉铅实验室。在经过连续的益生菌鉴定后，发现了26个实验室的存活。这26个益生菌在抗金属和去除金属的能力上表现出显著的差异。在26株细菌中，有7株(Cd 54-2、Cd 61-7、Cd 69-12、Cd 70-13、Pb82-8、Pb96-19和Cd 109-16)和4株(Pb71-1、Pb73-2、Pb85-9和Pb96-19)对水中镉和铅的去除效率分别高于其余菌株。在筛选的菌株中，菌株CD70-13和Pb71-1对MRS(De Man、Rogosa和Sharpe)培养基中镉的去除能力最高(25%)，对铅的去除能力(59%)最高，对鱼粘液具有良好的粘附性。对它们的16S rDNA序列的系统发育分析表明，菌株CD70-13和Pb71-1属于鲁氏乳杆菌。结论：新分离到的乳酸菌具有良好的益生菌特性、金属吸附特性和黏附特性，表明它们在肠道环境中具有较高的生存潜力和从环境中吸收受试金属的能力。这项研究的意义和影响：据我们所知，这是第一项旨在分离、表征和鉴定具有抗金属能力的实验室菌株的研究，这些菌株有可能成为食物中的益生菌候选者，并在鱼类肠道环境中进行体内挑战研究，以吸收和控制重金属的生物积累。
Aim: To isolate and characterize lactic acid bacteria (LAB) and determine whether they could potentially be used as heavy metal (cadmium and lead) absorbing probiotics. Methods and Results: The study used 53 environmental (mud and sludge) samples to isolate cadmium- and lead-resistant LAB, by following spared plate technique. A total of 255 cadmium- and lead-resistant LAB were isolated from these samples. The survival of 26 of the LAB was found after passing through sequential probiotic characterizations. These 26 probiotic LAB exhibited remarkable variations in their metal-resistant and metal-removal abilities. Of 26, seven (Cd54-2, Cd61-7, Cd69-12, Cd70-13, Pb82-8, Pb96-19 and Cd109-16) and four (Pb71-1, Pb73-2, Pb85-9 and Pb96-19) strains displayed relatively elevated cadmium- and lead-removal efficiencies from water, respectively, compare with that of the remaining strains. Strains Cd70-13 and Pb71-1 showed the highest cadmium (25%) and lead (59%) removal capacity from MRS (De Man, Rogosa and Sharpe) culture medium, respectively, amongst the selected strains and showed a good adhesive ability on fish mucus. A phylogenetic analysis of their 16S rDNA sequences revealed that the strains Cd70-13 and Pb71-1 belong to Lactobacillus reuteri. Conclusion: Excellent probiotic, metal sorption and adhesive characteristics of newly identified Lact.similar to reuteri strains Cd70-13 and Pb71-1 were isolated, which indicated their high potential abilities to survive in the intestinal milieu and to uptake the tested metals from the environment. Significance and Impact of the Study: To our knowledge, this is the first study that has aimed to isolate, characterize and identify metal-resistant LAB strains that have potential to be a probiotic candidate for food and in vivo challenge studies in the intestinal milieu of fish for the uptake and control of heavy metal bioaccumulation.