Phylogenetic analysis of Portuguese Feline Immunodeficiency Virus sequences reveals high genetic diversity
Phylogenetic analysis of Portuguese Feline Immunodeficiency Virus sequences reveals high genetic diversity
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DOI：
10.1016/j.vetmic.2005.11.056
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发表时间：
2006-04-16
影响因子：
3.3
通讯作者：
Tavares, L
中科院分区：
文献类型：
--
作者：
Duarte, A;Tavares, L
猫免疫缺陷病毒(FIV)是一种慢病毒，可引起家猫的一种免疫缺陷样疾病。根据Em的V3-V5区的遗传多样性，将FIV基因分为A、B、C、D和E五个系统发育亚型，并在世界范围内分布。为了了解FIV在葡萄牙的亚型多样性，在葡萄牙里斯本兽医学院医院进行了为期一年的血清学调查，以鉴定血清阳性动物。用套式聚合酶链式反应扩增了病毒的两个基因组区域，并进行了测序，并分析了24个新的葡萄牙FIV序列与其他已发表的FIV分离株之间的系统发育关系。这些序列的引入导致了B亚型的亚群，包括大多数新的葡萄牙序列。此外，出现了一个新的簇，具有两个高度差异的新序列，可能代表一个新的亚型。对这些新的FIV分离株的研究表明，在葡萄牙，B亚型内存在独特的病毒种群亚群，并且存在明显不同于五个已知亚型的序列，这为理解FIV的遗传多样性提供了贡献。(C)2005 Elsevier B.V.保留所有权利。
Feline Immunodeficiency Virus (FIV) is a Lentivirus responsible for an immunodeficiency like disease in domestic cats. Based on the genetic diversity of the V3-V5 region of em, gene FIV is divided in five phylogenetic subtypes (A, B, C, D and E) with a world-wide distribution. To understand the subtype diversity of FIV in Portugal a serological survey was conducted during 1 year in the Veterinary Faculty Hospital, Lisbon, Portugal to identify seropositive animals. Two viral genomic regions were amplified by a nested PCR, sequenced and the phylogenetic relationships between 24 new Portuguese FIV sequences and other previously published FIV isolates were assessed. The introduction of these sequences induced a subclustering in subtype B including most of the new Portuguese sequences. Moreover, a new cluster emerged, with two highly divergent new sequences that might represent a new subtype. The study of these new FIV isolates showed the presence in Portugal of a unique viral population subclustering within subtype B and of sequences clearly divergent from the five known subtypes, providing a contribution for the understanding of FIV's genetic diversity. (c) 2005 Elsevier B.V. All rights reserved.