DOC2 - A NOVEL BRAIN PROTEIN HAVING 2 REPEATED C-2-LIKE DOMAINS
DOC2 - A NOVEL BRAIN PROTEIN HAVING 2 REPEATED C-2-LIKE DOMAINS
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DOI：
10.1006/bbrc.1995.1062
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发表时间：
1995-01-17
影响因子：
3.1
通讯作者：
TAKAI, Y
中科院分区：
文献类型：
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作者：
ORITA, S;SASAKI, T;TAKAI, Y
在synaptotagmin和Rabphilin-3A中发现了与Ca2+和磷脂相互作用的两个重复的c -2样结构域，它们与神经递质释放有关。在这里，我们从人脑cDNA文库中分离出一种编码具有两个重复c -2样结构域的新蛋白的cDNA。分离的cDNA编码一个含有400个氨基酸的蛋白质，Mr为44,071。纯化的重组蛋白确实与Ca2+和磷脂相互作用。我们将这种蛋白命名为Doc2 （Double C-2）。Doc2仅在脑内表达，在突触囊泡部分高度集中。这些结果表明Doc2是一种新的脑蛋白，在神经递质释放中起Ca2+传感器的作用。(C) 1995学术出版社，Inc。
Two repeated C-2-like domains interacting with Ca2+ and phospholipid are found in synaptotagmin and Rabphilin-3A which are implicated in neurotransmitter release. Here we have isolated a cDNA encoding a novel protein having two repeated C-2-like domains from a human brain cDNA library. The isolated cDNA encodes a protein with 400 amino acids and a Mr of 44,071. The purified recombinant protein indeed interacts with Ca2+ and phospholipid. We have named this protein Doc2 (Double C-2). Doc2 is exclusively expressed in brain and is highly concentrated in the synaptic vesicle fraction. These results suggest that Doc2 is a novel brain protein and serves as a Ca2+ sensor in neurotransmitter release. (C) 1995 Academic Press, Inc.