DNA synthesis in the human diploid cell strain WI-38 during in vitro aging: an autoradiography study.
DNA synthesis in the human diploid cell strain WI-38 during in vitro aging: an autoradiography study.
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体外老化过程中人二倍体细胞株 WI-38 的 DNA 合成：放射自显影研究。
DOI：
10.1093/geronj/34.3.323
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
L. Hayflick
中科院分区：
文献类型：
--
作者：
T. Matsumura;E. Pfendt;L. Hayflick
WI-38细胞群中未掺入氚标记胸苷（3 H-TdR）的细胞百分比通过放射自显影术测定，测量未标记细胞核的百分比和3 H-TdR暴露开始和终止时的总细胞数比。该百分比受影响标记细胞增殖的因素影响最小，但取决于群体倍增水平（PDL）。结果表明，在增殖的WI-38群体中，存在S期进入速率极慢的亚群。该参数可用于凭经验估计细胞群体的倍增潜力。
The percent of cells in a WI-38 cell population which did not incorporate tritiated thymidine (3H-TdR) was determined by autoradiography from the measurement of percent nonlabeled nuclei and the ratio of total cell numbers at the initiation and at the termination of exposure to 3H-TdR. This percentage was minimally affected by factors influencing the proliferation of labeled cells, but was dependent on the population doubling level (PDL). The results suggest the presence, in a proliferating WI-38 population, of subpopulation(s) with an extremely slow rate of S phase entrance. The parameter was useful in estimating, empirically, the doubling potential of a cell population.