Biodegradation of 2,4,6-trichlorophenol in a packed-bed biofilm reactor equipped with an internal net draft tube riser for aeration and liquid circulation.
Biodegradation of 2,4,6-trichlorophenol in a packed-bed biofilm reactor equipped with an internal net draft tube riser for aeration and liquid circulation.
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DOI：
10.1016/j.jhazmat.2008.04.077
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发表时间：
2009-01
影响因子：
13.6
通讯作者：
A. Jesus;F. J. Romano-Baez;L. Leyva-Amezcua;C. Juárez-ramírez;N. Ruiz-Ordaz;J. Galíndez‐Mayer
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文献类型：
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作者：
A. Jesus;F. J. Romano-Baez;L. Leyva-Amezcua;C. Juárez-ramírez;N. Ruiz-Ordaz;J. Galíndez‐Mayer
为研究杀菌剂和落叶剂2，4，6-三氯苯酚（2，4，6-TCP）的好氧生物降解，构建了一个小型的带导流管液体循环充氧提升管的填充床生物反应器（PB-ALR）。为了获得相对于整个生物反应器体积的高填充床体积，使用高AD/AR比。反应堆的降液管里塞满了多孔的火山石碎片。评价PB-ALR流体力学和氧传质行为，并与作为内部气升式反应器（ALR）操作的未填充反应器的观察到的行为进行比较。总的气含率值ECOG，并在PB-ALR中的各种气流速度确定的区域氧传质系数，高于与ALR获得的。当比较在两种情况下获得的混合时间值时，当反应器作为PB-ALR操作时，观察到混合时间略微增加。采用生物膜反应器，采用混合菌群法对2，4，6-TCP的好氧生物降解进行了研究。三种细菌菌株被鉴定为伯克霍尔德氏菌属，Burkholderia kururiensis和Stenotrophomonas sp.组成的微生物群落能够共代谢降解2，4，6-TCP，使用苯酚作为主要底物。该联合体去除了100%的苯酚和近99%的2，4，6-TCP。矿化和脱卤的2，4，6-TCP被证明是高COD去除率（约95%），并从卤代化合物的化学计量释放氯离子（约80%）。最后，观察到微生物聚生体也能够在没有苯酚作为主要底物的情况下代谢2，4，6-TCP，具有高去除效率（2，4，6-TCP接近100%，COD为92%，氯离子为88%）。
For the aerobic biodegradation of the fungicide and defoliant 2,4,6-trichlorophenol (2,4,6-TCP), a bench-scale packed-bed bioreactor equipped with a net draft tube riser for liquid circulation and oxygenation (PB-ALR) was constructed. To obtain a high packed-bed volume relative to the whole bioreactor volume, a high AD/ARratio was used. Reactor's downcomer was packed with a porous support of volcanic stone fragments. PB-ALR hydrodynamics and oxygen mass transfer behavior was evaluated and compared to the observed behavior of the unpacked reactor operating as an internal airlift reactor (ALR). Overall gas holdup values ɛG, and zonal oxygen mass transfer coefficients determined at various airflow rates in the PB-ALR, were higher than those obtained with the ALR. When comparing mixing time values obtained in both cases, a slight increment in mixing time was observed when reactor was operated as a PB-ALR. By using a mixed microbial community, the biofilm reactor was used to evaluate the aerobic biodegradation of 2,4,6-TCP. Three bacterial strains identified as Burkholderia sp., Burkholderia kururiensis and Stenotrophomonas sp. constituted the microbial consortium able to cometabolically degrade the 2,4,6-TCP, using phenol as primary substrate. This consortium removed 100% of phenol and near 99% of 2,4,6-TCP. Mineralization and dehalogenation of 2,4,6-TCP was evidenced by high COD removal efficiencies (≈95%), and by the stoichiometric release of chloride ions from the halogenated compound (≈80%). Finally, it was observed that the microbial consortium was also capable to metabolize 2,4,6-TCP without phenol as primary substrate, with high removal efficiencies (near 100% for 2,4,6-TCP, 92% for COD and 88% for chloride ions).