Jasmonic acid-responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua
Jasmonic acid-responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua
复制标题
茉莉酸响应的 AabHLH1 正向调节青蒿中的青蒿素生物合成
DOI：
10.1002/bab.1733
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发表时间：
2019-05-01
影响因子：
2.8
通讯作者：
Tang, Kexuan
中科院分区：
文献类型：
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作者：
Li, Ling;Hao, Xiaolong;Tang, Kexuan
青蒿是倍半萜类青蒿素的唯一天然来源，后者被广泛用于治疗疟疾。植物激素茉莉酸（jasmonic acid， JA）能显著促进黄花蒿中青蒿素的合成。AabHLH1可以结合并激活青蒿素生物合成基因，如AaADS和AaCYP71AV1。在本研究中，我们证明了AabHLH1对MeJA处理有反应，并且在腺毛状体富集的组织中高表达，其表达谱与AaADS相似。酵母双杂交实验表明，AabHLH1与黄花酵母中9种AaJAZ蛋白均有相互作用。转基因植株功能分析表明，青蒿素合成基因在AabHLH1-OE转基因黄花蒿中表达上调，在AabHLH1-EAR转基因黄花蒿中表达下调；青蒿素在AabHLH1-OE系中含量升高，在AabHLH1-EAR系中含量降低。这些结果表明，ja诱导的AabHLH1通过调节生物合成基因正向调节青蒿素的生物合成，从而为ja诱导的黄花蒿青蒿素生物合成的调控机制提供了新的认识。
Artemisia annua is the only natural source of the sesquiterpenoid artemisinin, which is widely used to treat malaria. The phytohormone jasmonic acid (JA) can significantly promote artemisinin biosynthesis in A. annua. AabHLH1 can bind and activate artemisinin biosynthetic genes, such as AaADS and AaCYP71AV1. In this study, we proved that AabHLH1 was responsive to MeJA treatment and highly expressed in glandular trichome-enriched tissues, and that its expression profile was similar to that of AaADS. Yeast two-hybrid assays showed that AabHLH1 interacted with all nine AaJAZ proteins in A. annua. Functional analysis with transgenic plants showed that several artemisinin biosynthetic genes were upregulated in AabHLH1-OE transgenic A. annua lines and downregulated in AabHLH1-EAR lines; furthermore, the artemisinin content was increased in the AabHLH1-OE lines and decreased in the AabHLH1-EAR lines. These results demonstrate that the JA-induced AabHLH1 positively regulates artemisinin biosynthesis by regulating the biosynthetic genes, and thus provide new insight into the regulatory mechanism of JA-induced artemisinin biosynthesis in A. annua.