SECD, A NEW GENE INVOLVED IN PROTEIN EXPORT IN ESCHERICHIA-COLI
SECD, A NEW GENE INVOLVED IN PROTEIN EXPORT IN ESCHERICHIA-COLI
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DOI：
10.1128/jb.169.3.1286-1290.1987
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发表时间：
1987-03-01
影响因子：
3.2
通讯作者：
BECKWITH, J
中科院分区：
文献类型：
--
作者：
GARDEL, C;BENSON, S;BECKWITH, J
在phoA-lacZ和lamB-lacZ基因融合菌株中，通过搜索显示改变的乳糖表型的突变体，鉴定了蛋白质输出改变的大肠杆菌的新突变体。在一个新的基因secD中定位了几个突变。这些突变体，在一般情况下，冷敏感的增长，和突变导致出口蛋白质的前体的积累。secD基因与E.大肠杆菌染色体，但可从另一个基因提出参与出口，ssaD，它附近的地图。鉴定携带secD+的质粒并用于显示突变是隐性的。secD基因可以编码细胞输出机制的组分。
New mutants of Escherichia coli altered in protein export were identified in phoA-lacZ and lamB-lacZ gene fusion strains by searching for mutants that showed an altered lactose phenotype. Several mutations mapped in a new gene, secD. These mutants were, in general, cold sensitive for growth, and the mutations led to an accumulation of precursor of exported proteins. The secD gene is closely linked to tsx on the E. coli chromosome, but separable from another gene proposed to the involved in export, ssaD, which maps nearby. A plasmid carrying secD+ was identified and used to show that the mutations are recessive. The secD gene may code for a component of the cellular export machinery.