PROTEINS IN THE DEGENERATIVE RETINA OF C3H MICE: DEFICIENCY OF A CYCLIC‐NUCLEOTIDE PHOSPHODIESTERASE AND OPSIN 1
PROTEINS IN THE DEGENERATIVE RETINA OF C3H MICE: DEFICIENCY OF A CYCLIC‐NUCLEOTIDE PHOSPHODIESTERASE AND OPSIN 1
复制标题
C3H 小鼠退化视网膜中的蛋白质：环核苷酸磷酸二酯酶和视蛋白 1 的缺乏
作者：
D. Farber;R. Lolley
翻译后摘要-遗传性失明（C3 H/HeJ）小鼠视网膜的蛋白质模式进行了比较，从正常（DBA/1 J）视网膜在出生后的成熟。在十二烷基硫酸钠中通过聚丙烯酰胺凝胶电泳确定的蛋白质条带的数量在正常视网膜中随着发育而增加，并且到成年时，观察到30条蛋白质条带，分子量范围为13，500至105，000道尔顿。视网膜蛋白质中有4条为组蛋白，1条为微管蛋白。来自未成熟C3 H视网膜的蛋白质条带的模式与来自DBA视网膜的蛋白质条带的模式相似，但是到了成年期，来自C3 H视网膜的蛋白质条带的模式在三条蛋白质条带中缺乏，其中两条被鉴定为视蛋白和环核苷酸磷酸二酯酶。
Abstract— The protein patterns from retinae of mice with inherited blindness (C3H/HeJ) were compared with those from normal (DBA/1J) retinae during postnatal maturation. The number of protein bands, as determined by polyacrylamide gel electrophoresis in sodium dodecyl sulphate, increased in the normal retina with development and, by adulthood, 30 bands of protein were observed, with molecular weights ranging from 13,500 to 105,000 daltons. Four bands of retinal protein were suggested to be histones, and one was identified as tubulin. The patterns of protein bands from the immature C3H retinae were similar to those from the DBA retinae but, by adulthood, the pattern from the C3H retina was deficient in three bands of protein, two of which were identified as opsin and a cyclic‐nucleotide phosphodiesterase.