SOME PROPERTIES OF HEPATIC REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE - CYTOCHROME-C REDUCTASE
SOME PROPERTIES OF HEPATIC REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE - CYTOCHROME-C REDUCTASE
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DOI：
10.1021/bi00736a018
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发表时间：
1973-01-01
期刊：
影响因子：
2.9
通讯作者：
MASON, HS
中科院分区：
文献类型：
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作者：
IYANAGI, T;MASON, HS
Takashi Iyanagi J和H. S. Mason* 摘要：用胰蛋白酶溶解和层析法从兔肝微粒体中纯化出一种NADPH-细胞色素c还原酶，它具有以下性质：（1）每一多肽含有两个黄素，分子量为6.8 × 10 ~ 4（十二烷基硫酸钠凝胶电泳）或7.9 X 104（2）一种黄素为FAD，另一种黄素为FMN，二者的摩尔浓度大致相等;（3）在02存在下通过NADPH还原时，每一个含有一个黄素自由基的02稳定的中性半醌被还原。
Takashi IyanagiJ and H. S. Mason* abstract: A purified NADPH-cytochrome c reductase prepared by trypsin solubilization and chromatography from rabbit liver microsomes had the following properties:(1) it contained two flavines per single polypeptide of mol wt 6.8 X 104 (sodium dodecyl sulfate gel electrophoresis) or 7.9 X 104 (sedimentation equilibrium);(2) one flavine was FAD, the other, FMN, approximately equimolar;(3) upon reduction by NADPH in the presence of 02, an 02-stable neutral semiquinone containing one flavine free radical per