UDP-glucosyltransferase regulates grain size and abiotic stress tolerance associated with metabolic flux redirection in rice
UDP-glucosyltransferase regulates grain size and abiotic stress tolerance associated with metabolic flux redirection in rice
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DOI：
10.1038/s41467-020-16403-5
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发表时间：
2020-05-26
影响因子：
16.6
通讯作者：
Lin, Hong-Xuan
中科院分区：
文献类型：
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作者：
Dong, Nai-Qian;Sun, Yuwei;Lin, Hong-Xuan
籽粒大小是粮食产量的重要构成性状，经常受到非生物胁迫的威胁。然而，关于谷物产量和非生物胁迫耐受性是如何调控的，人们知之甚少。在这里，我们描述了GSA1，一个调节籽粒大小和与代谢通量重定向相关的非生物胁迫耐受性的数量性状基因座(QTL)。GSA1编码一种UDP-葡萄糖基转移酶，该酶对类黄酮和单甘醇具有葡萄糖转移酶活性。GSA1通过调节细胞的增殖和膨胀来调节颗粒的大小，而细胞的增殖和膨胀受类黄酮介导的生长素水平和相关基因表达的调节。在非生物胁迫下，GSA1参与了从木质素生物合成到类黄酮生物合成的代谢流的重定向，以及保护水稻免受非生物胁迫的类黄酮苷的积累。GSA1的过表达导致更大的颗粒和增强的非生物胁迫耐受性。我们的发现为调节与代谢通量重定向相关的颗粒大小和非生物胁迫耐受性提供了洞察，并为改良作物提供了潜在的手段。提高粮食产量需要放在环境压力的背景下考虑。在这里，作者揭示了UDP-葡萄糖基转移酶与水稻籽粒大小的调节、非生物胁迫耐受性、类黄酮介导的生长素信号以及碳流重定向到类黄酮苷的合成有关。
Grain size is an important component trait of grain yield, which is frequently threatened by abiotic stress. However, little is known about how grain yield and abiotic stress tolerance are regulated. Here, we characterize GSA1, a quantitative trait locus (QTL) regulating grain size and abiotic stress tolerance associated with metabolic flux redirection. GSA1 encodes a UDP-glucosyltransferase, which exhibits glucosyltransferase activity toward flavonoids and monolignols. GSA1 regulates grain size by modulating cell proliferation and expansion, which are regulated by flavonoid-mediated auxin levels and related gene expression. GSA1 is required for the redirection of metabolic flux from lignin biosynthesis to flavonoid biosynthesis under abiotic stress and the accumulation of flavonoid glycosides, which protect rice against abiotic stress. GSA1 overexpression results in larger grains and enhanced abiotic stress tolerance. Our findings provide insights into the regulation of grain size and abiotic stress tolerance associated with metabolic flux redirection and a potential means to improve crops. Increasing grain yield needs to be put in the context of environmental stress. Here, the authors reveal that a UDP-glucosyltransferase is associated with regulation of rice grain size, abiotic stress tolerance, flavonoid-mediated auxin signaling, and redirection of carbon flux to flavonoid glycosides synthesis.