Recycling of phase components during lysozyme extraction from hen egg white in the EO50PO50/K2HPO4 aqueous two-phase system
Recycling of phase components during lysozyme extraction from hen egg white in the EO50PO50/K2HPO4 aqueous two-phase system
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DOI：
10.1016/j.bej.2010.04.011
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发表时间：
2010-08-15
影响因子：
3.9
通讯作者：
Jankowski, T.
中科院分区：
文献类型：
--
作者：
Dembczynski, R.;Bialas, W.;Jankowski, T.
由热分离共聚物 EO50PO50 [50% (w/w) 环氧乙烷和 50% (w/w) 环氧丙烷] 和 K2HPO4 组成的水性两相系统 (ATPS) 用于通过四次连续提取从鸡蛋清中提取溶菌酶，并利用相成分的回收和再循环。提取分两个阶段进行。在第一阶段，形成了 40% EO50PO50/10% K-2 HPO4 (w/w) 的主要两相系统，溶菌酶分配到顶部富含共聚物的相，而污染物蛋白质保留在底部富含磷酸盐的相中。在下一阶段中，分离富含共聚物的相并进行温度诱导的相分离。所得的二次两相体系由共聚物的浓溶液和溶菌酶的水溶液组成。接下来，将热分离后回收的EO50PO50浓缩液与从初级系统回收的磷酸盐溶液混合，并加入新的鸡蛋清，得到新的溶菌酶提取系统。在四次连续提取过程中获得的溶菌酶制剂的比活性范围为 38,438 至 42,907 U/mg 蛋白质。 (C) 2010 Elsevier B.V. 保留所有权利。
An aqueous two-phase system (ATPS) composed of a thermoseparating copolymer EO50PO50 [50% (w/w) ethylene oxide and 50% (w/w) propylene oxide] and K2HPO4 was used to extract lysozyme from hen egg white over four successive extractions, using the recovery and recycling of phase components. Extraction was performed in two stages. In the first stage the primary two-phase system of 40% EO50PO50/10% K-2 HPO4 (w/w) was formed and the lysozyme partitioned to the top copolymer-rich phase while the contaminant proteins remained in the bottom phosphate-rich phase. In the next stage the copolymer-rich phase was isolated and subjected to temperature-induced phase separation. The resultant secondary two-phase system was composed of a concentrated solution of copolymer and a water solution of lysozyme. Next, the concentrated solution of EO50PO50 recovered after thermoseparation was mixed with the phosphate solution recovered from the primary system and a new portion of hen egg white added to obtain a new lysozyme extraction system. The specific activity of lysozyme preparations obtained during the four successive extractions ranged from 38,438 to 42,907 U/mg of protein. (C) 2010 Elsevier B.V. All rights reserved.