Stationary phase thickness determines the quality of thin-layer chromatography/matrix-assisted laser desorption and ionization mass spectra of lipids.
Stationary phase thickness determines the quality of thin-layer chromatography/matrix-assisted laser desorption and ionization mass spectra of lipids.
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DOI：
10.1016/j.ab.2014.02.002
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发表时间：
2014-04
影响因子：
2.9
通讯作者：
Hans Griesinger;B. Fuchs;R. Süss;Katerina Matheis;M. Schulz;J. Schiller
中科院分区：
文献类型：
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作者：
Hans Griesinger;B. Fuchs;R. Süss;Katerina Matheis;M. Schulz;J. Schiller
正相薄层色谱法（NP TLC）是一种成熟的（磷）脂分析方法。然而，通过NP TLC测定脂肪酰基组成是一项更具挑战性的任务。TLC分离与质谱检测的直接耦合（例如，基质辅助激光解吸/电离质谱法（MALDIMS）能够详细表征复杂的脂质混合物。在这里，我们表明，硅胶层的厚度有相当大的影响直接从TLC板记录的质谱的质量。特别地，如果使用“更薄”的TLC层，则可以降低基质背景信号的强度。
Normal phase thin-layer chromatography (NP TLC) is an established method of (phospho)lipid analysis. The determination of the fatty acyl composition is, however, a more challenging task by NP TLC. The direct coupling of TLC separation with mass spectrometric detection (e.g., matrix-assisted laser desorption/ionization mass spectrometry, MALDI MS), however, enables a detailed characterization of complex lipid mixtures. Here we show that the thickness of the silica gel layer has a considerable effect on the quality of the mass spectra recorded directly from the TLC plate. In particular, the intensity of the matrix background signals can be reduced if “thinner” TLC layers are used.