Improving Cellular Properties for Genetic Manipulation by Dispersed Growing Mutagenesis in Myxococcus fulvus HW-1
Improving Cellular Properties for Genetic Manipulation by Dispersed Growing Mutagenesis in Myxococcus fulvus HW-1
复制标题
通过黄粘球菌 HW-1 分散生长诱变改善遗传操作的细胞特性
DOI：
10.1007/s00284-009-9554-0
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发表时间：
2010-06
期刊：
影响因子：
--
通讯作者：
Zhang Cuiying
中科院分区：
文献类型：
--
作者：
Li Yuezhong;Cai ke;Wu Zhihong;Zhang Cuiying
粘细菌基因操作的一个关键限制是细胞在液体中成团生长。以黄色粘球菌HW-1为出发菌株，经紫外线诱变，获得一株完全分散生长的突变株UV 684。亲本HW-1和突变体UV 684的耐盐性差异不显著。突变株UV 684与亲本菌株具有相似的子实体形成能力和S运动能力。有趣的是，该突变体表现出高转化/转座效率，每微克DNA具有10（5）-10（6）菌落形成单位，这比HW-1高约10（3）-10（5）倍。结果表明，导致UV 684突变株分散生长的突变对社会行为有一些影响，但大大促进了分子遗传操作。
One of the key limitations to genetic manipulation in myxobacteria is that the cells grow in clumps in liquid. A salt-tolerant strain HW-1 of Myxococcus fulvus was treated with UV irradiation and produced a completely dispersedly growing mutant UV684. There were no significant differences between the parent HW-1 and the mutant UV684 in terms of salt-tolerant growth. The mutant UV684 and the parent strain had the similar abilities of the fruiting body formation and S motility. Interestingly, the mutant exhibited high transformation/transposition efficiency with 10(5)-10(6) colony-forming units per microg DNA, which was about 10(3)-10(5) fold higher than HW-1. The results indicate that the mutation that led to dispersed growth in the UV684 mutant strain had a few impacts on social behavior, but greatly facilitated molecular genetic manipulation.
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影响因子：
3.6
作者：
KASHEFI, K;HARTZELL, PL
通讯作者：
HARTZELL, PL
影响因子：
3.6
作者：
B. Wang;Wei Hu;Hong Liu;Cui-ying Zhang;Jing-yi Zhao;De-ming Jiang;Zhi-hong Wu;Yue-zhong Li
通讯作者：
B. Wang;Wei Hu;Hong Liu;Cui-ying Zhang;Jing-yi Zhao;De-ming Jiang;Zhi-hong Wu;Yue-zhong Li
影响因子：
3.2
作者：
M. Ward;Kenny C. Mok;D. Astling;H. Lew;D. Zusman
通讯作者：
M. Ward;Kenny C. Mok;D. Astling;H. Lew;D. Zusman
DOI：
10.1073/pnas.95.21.12376
发表时间：
1998-10-13
影响因子：
11.1
作者：
Velicer, GJ;Kroos, L;Lenski, RE
通讯作者：
Lenski, RE
影响因子：
2.7
作者：
HAGEN, DC;BRETSCHER, AP;KAISER, D
通讯作者：
KAISER, D