Lgr5 marks cycling, yet long-lived, hair follicle stem cells
Lgr5 marks cycling, yet long-lived, hair follicle stem cells
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DOI：
10.1038/ng.239
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发表时间：
2008-11-01
期刊：
影响因子：
30.8
通讯作者：
Toftgard, Rune
中科院分区：
文献类型：
--
作者：
Jaks, Viljar;Barker, Nick;Toftgard, Rune
在小鼠毛囊中，凸起部分的一组静止细胞被认为具有干细胞活性。 Lgr5 是肠干细胞的标记物，在休止期毛囊的隆起和次生芽以及生长期毛囊的下部外根鞘中的活跃循环细胞中表达。在这里，我们表明Lgr5（+）细胞包含活跃增殖的多能干细胞群，能够产生新的毛囊并长期维持毛囊的所有细胞谱系。 Lgr5(+) 后代重新填充毛囊中的其他干细胞区室，支持干细胞或祖细胞层次结构的存在。通过在通过下部外根鞘的运输过程中标记 Lgr5(+) 细胞，我们表明这些细胞保留了干细胞特性，并有助于下一个毛发生长初期的毛囊生长。表达分析表明自分泌 Hedgehog 信号参与维持 Lgr5(+) 干细胞群。
In mouse hair follicles, a group of quiescent cells in the bulge is believed to have stem cell activity. Lgr5, a marker of intestinal stem cells, is expressed in actively cycling cells in the bulge and secondary germ of telogen hair follicles and in the lower outer root sheath of anagen hair follicles. Here we show that Lgr5(+) cells comprise an actively proliferating and multipotent stem cell population able to give rise to new hair follicles and maintain all cell lineages of the hair follicle over long periods of time. Lgr5(+) progeny repopulate other stem cell compartments in the hair follicle, supporting the existence of a stem or progenitor cell hierarchy. By marking Lgr5(+) cells during trafficking through the lower outer root sheath, we show that these cells retain stem cell properties and contribute to hair follicle growth during the next anagen. Expression analysis suggests involvement of autocrine Hedgehog signaling in maintaining the Lgr5(+) stem cell population.