Interferon tau-dependent and independent effects of the bovine conceptus on the endometrial transcriptome
Interferon tau-dependent and independent effects of the bovine conceptus on the endometrial transcriptome
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DOI：
10.1093/biolre/ioy199
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发表时间：
2019-02-01
影响因子：
3.6
通讯作者：
Lonergan, Patrick
中科院分区：
文献类型：
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作者：
Mathew, Daniel J.;Sanchez, Jose M.;Lonergan, Patrick
本研究研究了牛妊娠诱导的子宫内膜转录组修饰与干扰素tau（IFNT）、妊娠起源（体内与体外）和妊娠性别的影响相关。体外（IVF）或体内（超数排卵和人工授精，AI）产生的囊胚在发情周期的第7天转移到受体小母牛。在第15天，通过子宫冲洗获得IVF或AI衍生的孕体，并将其单独放置在培养基中的子宫内膜外植体上6小时。外植体也用单独的培养基作为对照或用含有100 ng/mL IFNT的培养基培养。通过RNA-Seq分析总外植体RNA。子宫内膜与IFNT或IVF或AI衍生的孕体的孵育改变（P
This study investigated bovine conceptus-induced modifications to the endometrial transcriptome related to effects of interferon tau (IFNT), conceptus origin (in vivo vs. in vitro), and conceptus sex. In vitro (IVF) or in vivo (superovulation and artificial insemination, AI) produced blastocysts were transferred into recipient heifers on day 7 of the estrous cycle. On day 15, IVF- or AI-derived conceptuses were obtained by uterine flushing and individually placed on endometrial explants in media for 6 h. Explants were also cultured with media alone as a control or media containing 100 ng/mL IFNT. Total explant RNA was analyzed by RNA-Seq. Incubation of endometrium with IFNT or IVF- or AI-derived conceptuses changed (P