Microfluidic array platform for simultaneous lipid bilayer membrane formation
Microfluidic array platform for simultaneous lipid bilayer membrane formation
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DOI：
10.1016/j.bios.2008.07.022
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发表时间：
2009-01-01
影响因子：
12.6
通讯作者：
Morgan, H.
中科院分区：
文献类型：
--
作者：
Zagnoni, M.;Sandison, M. E.;Morgan, H.
近年来，蛋白质芯片技术在蛋白质组学中得到了广泛的应用。然而，仍然需要用于蛋白质-蛋白质和蛋白质-化合物相互作用的高通量分析的新方法。在本文中，脂质双层膜阵列内形成的微流控系统与集成电极。该系统由夹在一起的三层组成，因此使器械可清洁和可重复使用。该装置微流体能够使用先前开发的空气暴露技术同时形成脂质双层阵列，从而避免手动形成单个双层的需要。Ag/AgCl电极允许离子通道测量，每个位点都是独立可寻址的。通常，在12个位点上同时形成脂质双层的产率为50%，并且在多个位点上获得了离子通道记录。该系统具有很大的潜力，悬浮脂质双分子层阵列的自动化平台的发展。(C)2008 Elsevier B. V.保留所有权利。
In recent years, protein array technologies have found widespread applications in proteomics. However, new methods for high-throughput analysis of protein-protein and protein-compound interactions are still required. In this paper, an array of lipid bilayer membranes formed within a microfluidic system with integrated electrodes is presented. The system is comprised of three layers that are clamped together, thus rendering the device cleanable and reusable. The device microfluidics enable the simultaneous formation of an array of lipid bilayers using a previously developed air-exposure technique, thereby avoiding the need to manually form individual bilayers. The Ag/AgCl electrodes allow for ion channel measurements, each of the sites being independently addressable. Typically, a 50% yield in simultaneous lipid bilayer formation over 12 sites was obtained and ion channel recordings have been acquired over multiple sites. This system has great potential for the development of an automatable platform of suspended lipid bilayer arrays. (C) 2008 Elsevier B.V. All rights reserved.