Influences of up-regulation of miR-126 on septic inflammation and prognosis through AKT/Rac1 signaling pathway
Influences of up-regulation of miR-126 on septic inflammation and prognosis through AKT/Rac1 signaling pathway
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DOI：
10.26355/eurrev_201903_17257
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发表时间：
2019-03-01
影响因子：
3.3
通讯作者：
Li, Y.
中科院分区：
文献类型：
--
作者：
Wang, H-F;Wang, Y-Q;Li, Y.
目的：为探讨微核糖核酸（microribonucleic acid，miR）-126上调通过AKT/Rac 1信号通路对脓毒症炎症及预后的影响，采用体外培养人肺微血管内皮细胞（Human pulmonary microvascular endothelial cells，HMVECs），转染miR-126模拟物。将处于对数生长期的HMVECs与凝血酶共同孵育。采用电细胞-基质阻抗传感（ECIS）系统检测HMVECs的跨膜电阻率作为渗透性。通过免疫荧光观察内皮细胞间隙。建立脓毒症小鼠模型，提取血清检测白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）。根据死亡时间绘制存活曲线。采用SPSS 22.0软件进行统计分析，结果表明：
OBJECTIVE: To explore the influences of the up-regulation of micro ribonucleic acid (miR)-126 on septic inflammation and prognosis through the AKT/Rac1 signaling pathway.MATERIALS AND METHODS: Human pulmonary microvascular endothelial cells (HMVECs) were cultured and transfected with miR-126 mimics. The HMVECs in the logarithmic growth phase in different groups were incubated with thrombin. The transmembrane resistivity of HMVECs was detected as the permeability via Electric Cell-substrate Impedance Sensing (ECIS) system. The endothelial cell space was observed via immunofluorescence. The mouse model of sepsis was then established and the serum was extracted to detect interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha). The survival curve was plotted based on the death time. The Statistical Product and Service Solutions (SPSS) 22.0 was used for statistical analysis, and p