Vaccinia virus ketch protein A55 is a 64 kDa intracellular factor that affects virus-induced cytopathic effect and the outcome of infection in a murine intradermal model
Vaccinia virus ketch protein A55 is a 64 kDa intracellular factor that affects virus-induced cytopathic effect and the outcome of infection in a murine intradermal model
复制标题
DOI：
10.1099/vir.0.81854-0
复制
发表时间：
2006-06-01
影响因子：
3.8
通讯作者：
Smith, Geoffrey L.
中科院分区：
文献类型：
--
作者：
Beard, Philippa M.;Froggatt, Graham C.;Smith, Geoffrey L.
牛痘病毒（VACV）蛋白A55是一种BTB/kelch蛋白，在N-末端区域具有宽复合物、tramtrack和bric-a-brac（BTB）结构域，并且在C-末端一半具有5个kelch重复。Kelch蛋白超家族的BTB/Kelch亚组与包括细胞骨架调节在内的多种功能相关。VACV含有三个预测编码BTB/kelch蛋白的基因：A55 R、F3 L和C2 L。A55 R基因产物已被鉴定为在感染后期表达的64 kDa的细胞内蛋白。构建了缺乏93.6%的A55 R开放阅读框（v Delta A55）的VACV菌株，发现其体内生长速率不变，但斑块形态和细胞病变效应不同，VACV诱导的Ca 2+非依赖性细胞/细胞外基质粘附的发生减少。在鼠皮内VACV感染模型中，缺乏A55 R基因的病毒比野生型和回复突变对照病毒诱导更大的病变。
The vaccinia virus (VACV) protein A55 is a BTB/kelch protein with a broad-complex, tramtrack and bric-a-brac (BTB) domain in the N-terminal region and five kelch repeats in the C-terminal half. The BTB/kelch subgroup of the kelch superfamily of proteins has been associated with a wide variety of functions including regulation of the cytoskeleton. VACV contains three genes predicted to encode BTB/kelch proteins: A55R, F3L and C2L. The A55R gene product has been identified as an intracellular protein of 64 kDa that is expressed late in infection. A VACV strain lacking 93.6% of the A55R open reading frame (v Delta A55) was constructed and found to have an unaltered growth rate in vivo but a different plaque morphology and cytopathic effect, as well as reduced development of VACV-induced Ca2+-independent cell/extracellular matrix adhesion. In a murine intradermal model of VACV infection, a virus lacking the A55R gene induced larger lesions than wild-type and revertant control viruses.