FIGC, a novel FGF-induced ubiquitin-protein ligase in gastric cancers
FIGC, a novel FGF-induced ubiquitin-protein ligase in gastric cancers
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DOI：
10.1016/j.febslet.2004.10.071
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发表时间：
2004-12-03
期刊：
影响因子：
3.5
通讯作者：
Jang, JH
中科院分区：
文献类型：
--
作者：
Jang, JH
我们以前的研究表明，成纤维细胞生长因子受体2（FGFR 2）在胃癌发生中起着重要作用。在这项研究中，我们已经使用了差异显示的方法来确定碱性成纤维细胞生长因子（bFGF）诱导基因在胃癌细胞。在这里，我们报告说，这些基因之一，预计编码一个环指蛋白，指定FIGC。发现FIGC基因编码381个氨基酸的多肽，在NH 2-末端和COOH-末端富含脯氨酸的区域具有新的RING指模块。利用重组蛋白的体外泛素化测定，我们证明FIGC具有内在的E3泛素连接酶活性，并促进泛素化。我们的数据表明，FIGC的上调，在胃癌中的bFGF的反应可能涉及通过生长调节剂失调的致癌作用。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
We have previously shown that fibroblast growth factor receptor 2 (FGFR2) plays an important role in gastric carcinogenesis. In this study, we have used a differential display approach to identify basic fibroblast growth factor (bFGF)inducible genes in gastric cancer cells. Here, we report that one of these genes is predicted to encode a RING finger protein, designated FIGC. The FIGC gene was found to encode a polypeptide of 381 amino acids with a novel RING finger module at the NH2-terminus and the COOH-terminal proline-rich region. Using an in vitro ubiquitination assay with recombinant protein, we demonstrate that FIGC has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination. Our data indicate that FIGC upregulation in response to bFGF in gastric cancer might be implicated in carcinogenesis through dysregulation of growth modulator. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.