Genomic characterization and phylogenetic analysis of a new canine picornavirus variant in the mainland of China
Genomic characterization and phylogenetic analysis of a new canine picornavirus variant in the mainland of China
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DOI：
10.1016/j.virusres.2021.198351
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发表时间：
2021-02-28
期刊：
影响因子：
5
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Li, Chuanfeng;Li, Yeqiu;Wang, Yong
应用病毒基因组学方法，从中国大陆中国地区的犬粪标本中检出一株新的犬细小核糖核酸病毒变异株，命名为DOG/SH1901/CHN/2019，并对其近全基因组序列进行测定和分析。序列分析表明，它具有标准的微小核糖核酸病毒基因组结构，即在5？UTR中有一个I型内部核糖体进入位点(IRES)。然而，与密切相关的CanPV相比，DOG/SH1901/CHN/2019产生了一系列独特的AA突变和7AA插入。系统进化分析和基于P1、2C、3C和3D蛋白序列的比对结果表明，DOG/SH1901/CHN/2019与冠状病毒244 F、325 F和6D株亲缘关系较近，属于一个独立的进化支系，与微冠状病毒属的A128thr株有较远的亲缘关系，提示未归类的冠状病毒株可能属于短冠冠状病毒科新种或新属。这项研究扩大了我们对冠状静脉的进化和遗传多样性的认识。
A new canine picornavirus (CanPV) variant, designated as dog/SH1901/CHN/2019, was detected in a pool of various canine fecal samples in the mainland of China using a viral metagenomic analysis, and its nearly com-plete genome sequence was determined and analyzed. Sequence analyses revealed that it had a standard picornavirus genome organization, a type I internal ribosome entry site (IRES) in the 5?UTR. However, dog/ SH1901/CHN/2019 has generated a serial of unique aa mutations and 7aa insertion when compared with the closely related CanPVs. Phylogenetic analysis and pairwise sequence comparisons based on the P1, 2C, 3C, and 3D protein sequences showed that dog/SH1901/ CHN/2019 was closely related to CanPV strains 244 F, 325 F and 6D, which clustered into an independent evolutionary clade and distantly related to CanPV strain A128thr of the genus Mischivirus, which indicated the unclassified CanPV strains may belong to a novel species or genus in the family Picornaviridae. This study extends our knowledge on the evolution and genetic diversity of CanPVs.