CULTURES OF BOVINE TRACHEAL EPITHELIUM WITH DIFFERENTIATED ULTRASTRUCTURE AND ION-TRANSPORT
CULTURES OF BOVINE TRACHEAL EPITHELIUM WITH DIFFERENTIATED ULTRASTRUCTURE AND ION-TRANSPORT
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DOI：
10.1007/bf02634367
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发表时间：
1993-01-01
影响因子：
2.1
通讯作者：
WIDDICOMBE, JH
中科院分区：
文献类型：
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作者：
KONDO, M;FINKBEINER, WE;WIDDICOMBE, JH
气管上皮细胞在用人胎盘胶原包被的Nuclepore滤器上生长。当生长浸没在培养基中含有胎牛血清，细胞显示未分化的超微结构（无纤毛和细胞高度约10 μ m）。短路电流（I（sc））约为天然上皮的1/10。相比之下，当生长在与空气界面的无血清培养基中时，细胞显示I（SC）等于或大于原始组织，具有纤毛，并具有约50 μ m的细胞高度。在I（SC）介质的反应是类似的原始组织，但不同的狗或人气管上皮。由于天然组织的易得性和低成本，在无血清培养基中生长的牛气管培养物与空气界面应证明在气道上皮生理学的研究中是有用的。
Tracheal epithelial cells were grown on Nuclepore filters coated with human placental collagen. When grown immersed in medium containing fetal bovine serum, cells displayed an undifferentiated ultrastructure (no cilia and a cell height of approximately 10 mum). Short-circuit current (I(sc)) was approximately 1/10 that of the native epithelium. By contrast, when grown in hormonally defined, serum-free medium with an air interface, cells showed I(sc) equal to or greater than the original tissue, possessed cilia, and had a cell height of approximately 50 mum. Responses in I(sc) to mediators were similar to those of the original tissue, but differed from those of dog or human tracheal epithelium. Given the ready availability and low cost of the native tissues, bovine tracheal cultures grown in serum-free medium with an air interface should prove useful in studies of airway epithelial physiology.