High-pressure-mediated dissociation of immune complexes demonstrated in model systems.
High-pressure-mediated dissociation of immune complexes demonstrated in model systems.
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在模型系统中证明了高压介导的免疫复合物解离。
DOI：
10.1093/clinchem/44.2.299
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发表时间：
1998
影响因子：
9.3
通讯作者：
J. Laugharn
中科院分区：
文献类型：
--
作者：
C. Cheung;D. J. Green;G. Litt;J. Laugharn
在两种模型系统中证明了使用压力破坏免疫复合物：前列腺特异性抗原（PSA）和抗PSA抗体；表胰甘蛋白，一种粘蛋白糖蛋白，以及一种针对这种蛋白的抗体。在室温（约21℃）下施加415 MPa和550 MPa （1 MPa约144 psi）的压力时，观察到抗PSA抗体与固定化PSA抗原的解离。施加从140兆帕到550兆帕的压力导致抗体从表甘露聚糖解离。在这两种情况下，解离抗体与固定抗原的重新结合表明，高压对免疫复合物结合的影响是可逆的。这些发现表明，应用高静水压力有可能显著提高临床检测的敏感性和特异性。
The use of pressure to disrupt immune complexes was demonstrated in two model systems: prostate-specific antigen (PSA) and anti-PSA antibody; and epiglycanin, a mucin glycoprotein, and an antibody specific to that protein. Dissociation of the anti-PSA antibody from the immobilized PSA antigen was observed when pressures of 415 MPa and 550 MPa (1 MPa approximately 144 psi) were applied at room temperature (approximately 21 degrees C). Application of pressures ranging from 140 MPa to 550 MPa resulted in dissociation of antibody from epiglycanin. In both cases, the rebinding of dissociated antibody to immobilized antigen indicated that the effect of high pressure on the binding of the immune complexes was reversible. These findings suggest that application of high hydrostatic pressure has the potential to be used to significantly improve the sensitivity and specificity of clinical assays.
HIV 感染者体内人类免疫缺陷病毒 (HIV) 抗原、p24 抗体和含 HIV 免疫复合物循环水平的关系。
DOI：
10.1093/infdis/158.5.1088
发表时间：
1988
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
McHugh,TM;Stites,DP;Busch,MP;Krowka,JF;Stricker,RB;Hollander,H
通讯作者：
Hollander,H
前列腺特异性抗原与 α2-巨球蛋白复合物的定量免疫测定。
影响因子：
9.3
作者：
España,F;Sánchez-Cuenca,J;Estellés,A;Gilabert,J;Griffin,JH;Heeb,MJ
通讯作者：
Heeb,MJ