Auto nanomanipulation system for single cell mechanical property characterization inside an environmental SEM
Auto nanomanipulation system for single cell mechanical property characterization inside an environmental SEM
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DOI：
10.1109/iros.2012.6385731
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发表时间：
2012-12
期刊：
影响因子：
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通讯作者：
Yajing Shen;M. Nakajima;M. Homma;T. Fukuda
中科院分区：
文献类型：
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作者：
Yajing Shen;M. Nakajima;M. Homma;T. Fukuda
单细胞的力学特性表征对于理解细胞的状态和活性是重要的。本文提出了一种用于环境扫描电子显微镜（ESEM）中单细胞力学特性表征的自动纳米操纵系统。采用聚焦离子束刻蚀技术，在原子力显微镜悬臂梁上制作了纳米针。将纳米针固定到纳米机器人操纵器上，该操纵器具有三个自由度，即X、Y和Z平移。将单个酵母细胞置于ESEM内的钨探针基底上。纳米针和单个细胞的位置信息被用作反馈信号来控制纳米机器人操作器的运动。最后，使用由自动纳米操纵系统驱动的纳米针测量单细胞的刚度。
Single cell's mechanical property characterization is important for the understanding of the cell's condition and activity. In this paper, an automatic nanomanipulation system was proposed for single cell mechanical property characterization inside an environmental scanning electron microscopy (ESEM). A nanoneedle was fabricated from AFM (atomic force microscopy) cantilever by FIB (focused ion beam) etching technique. The nanoneedle was fixed to a nanorobotic manipulator, which has three degrees of freedom, i.e. X, Y and Z translation. Single yeast cell was put on an tungsten probe substrate inside ESEM. The position information of the nanoneedle and single cell were used as the feedback signals to control the movement of the nanorobotic manipulator. Finally, the stiffness of the single cell was measured using the nanoneedle driven by the automatic nanomanipulation system.