Regulation of defense responses via heat shock transcription factors in Cucumis sativus L. against Botrytis cinerea
Regulation of defense responses via heat shock transcription factors in Cucumis sativus L. against Botrytis cinerea
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DOI：
10.1007/s10327-021-01041-6
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发表时间：
2021-11-12
影响因子：
1.2
通讯作者：
Sato, Tatsuo
中科院分区：
文献类型：
--
作者：
Kharisma, Agung Dian;Arofatullah, Nur Akbar;Sato, Tatsuo
热休克转录因子（HSFs）调控植物对热胁迫的反应，但其在热休克诱导抗性（HSIR）中的功能尚不清楚。本研究通过图像处理和定量基因表达分析，研究了hsf在黄瓜（Cucumis sativus）热休克（HST, 50℃，20 s）诱导对灰霉菌（Botrytis cinerea）抗性中的作用。HST减少了病变面积的百分比，与过氧化物酶（POX）和致病相关（PR）的上调一致1。C1基因分别在处理后24和48 h表达。这两个基因是在热休克转录因子A2 （HSFA2）和B2 （HSFB2）基因上调后检测到的。热休克元件（HSE）基序位于POX、PR1上游。C1， POX73和POX43，并且在HST后24 h上调。热休克蛋白90 （HSP90）抑制剂格尔达霉素（GDA）维持了含hse PR的上调，从而减少了灰霉病变。GDA处理增强了局部抗性，而HST则诱导了全身抗性。热激和GDA联合处理可诱导含hse的PR表达，增强植物对绿僵菌的抗性。HSIR机制涉及与系统性获得性耐药（SAR）相关的途径。它还涉及hsf介导的途径，该途径与局部抗性相关，并被SAR隐藏。本研究为HSIR的分子机制提供了新的见解，并为激活黄瓜植物对病原体的防御反应提供了另一种方法。
Heat shock transcription factors (HSFs) regulate the responses of plants to heat stress, but their functions in heat-shock-induced resistance (HSIR) are poorly understood. Here, we investigated the functions of HSFs in the resistance induced by a heat shock treatment (HST; 50 degrees C for 20 s) against gray mold (Botrytis cinerea) in Cucumis sativus by measuring lesion area using image processing and quantitative gene expression analysis. The HST reduced the percentage of lesion area, consistent with the upregulation of peroxidase (POX) and pathogenesis-related (PR) 1.C1 genes at 24 and 48 h after treatment, respectively. Both genes were detected after the upregulation of the heat shock transcription factor A2 (HSFA2) and B2 (HSFB2 genes. Heat shock element (HSE) motifs were found upstream of POX, PR1.C1, POX73, and POX43, and they were upregulated at 24 h after the HST. A heat shock protein 90 (HSP90) inhibitor, geldanamycin (GDA), maintained the upregulation of the HSE-containing PR, thereby reducing gray mold lesions. The GDA treatment enhanced local resistance, whereas the HST induced systemic resistance. The combination of heat shock and GDA treatments induced HSE-containing PR expression and enhanced plant resistance against B. cinerea. The HSIR mechanism involves a pathway related to systemic acquired resistance (SAR). It also involves an HSF-mediated pathway that is associated with local resistance and concealed by the SAR. This study provides novel insights into the molecular mechanisms of HSIR and an alternative approach to activate defense responses against pathogens in cucumber plants.