Role of the neutrophil chemorepellent soluble dipeptidyl peptidase IV in decreasing inflammation in a murine model of arthritis.
Role of the neutrophil chemorepellent soluble dipeptidyl peptidase IV in decreasing inflammation in a murine model of arthritis.
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DOI：
10.1002/art.39250
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Gomer RH
中科院分区：
文献类型：
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作者：
Herlihy SE;Brown ML;Pilling D;Weeks BR;Myers LK;Gomer RH
确定关节内注射中性粒细胞化学驱避剂二肽基肽酶 IV（DPPIV；CD26）是否可以减轻小鼠模型中的炎症并降低关节炎的严重程度。 DBA/1小鼠用II型胶原/弗氏完全佐剂免疫以产生胶原诱导的关节炎(CIA)。免疫后第25天，关节内注射重组人DPPIV（rhDPPIV）或磷酸盐缓冲盐水，每周记录3次关节炎严重程度评分。将两组小鼠后腿固定、脱钙、石蜡包埋、切片。以 1-8 级记录炎症和中性粒细胞浸润的病理评分，并通过形态学细胞计数确定中性粒细胞的数量。此外，分别使用抗 Mac-2 抗体和组织学染色评估 Mac-2 阳性巨噬细胞和关节损伤。注射 rhDPPIV 可以降低 CIA 小鼠关节炎严重程度的平均评分。 DPPIV 治疗减少了关节炎关节周围和关节周围组织的炎症和关节损伤的总体程度，还减少了中性粒细胞和巨噬细胞的浸润。局部注射中性粒细胞化学驱避剂 DPPIV 可减轻关节炎小鼠模型中的炎症和疾病的严重程度。
To determine whether an intraarticular injection of the neutrophil chemorepellent dipeptidyl peptidase IV (DPPIV; CD26) can attenuate inflammation and decrease the severity of arthritis in a murine model. DBA/1 mice were immunized with type II collagen/Freund's complete adjuvant to produce collagen-induced arthritis (CIA). On day 25 postimmunization, recombinant human DPPIV (rhDPPIV) or phosphate buffered saline was injected intraarticularly, and arthritis severity scores were recorded 3 times per week. The hind legs of mice in both groups were fixed, decalcified, paraffin embedded, and sectioned. Pathologic scores for inflammation and neutrophil infiltration were recorded on a scale of 1–8, and the number of neutrophils was determined by morphometric cell counts. In addition, Mac-2–positive macrophages and articular damage were assessed using anti–Mac-2 antibodies and histologic staining, respectively. Injection of rhDPPIV reduced the mean score of arthritis severity in mice with CIA. DPPIV treatment reduced the overall extent of inflammation and articular damage around the arthritic joint and periarticular tissue, and also decreased neutrophil and macrophage infiltration. A localized injection of the neutrophil chemorepellent DPPIV reduces inflammation and the severity of the disease in a murine model of arthritis.