Endogenous acid ceramidase protects epithelial cells from Porphyromonas gingivalis-induced inflammation in vitro.
Endogenous acid ceramidase protects epithelial cells from Porphyromonas gingivalis-induced inflammation in vitro.
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DOI：
10.1016/j.bbrc.2017.12.137
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发表时间：
2018-01-22
影响因子：
3.1
通讯作者：
Movila A
中科院分区：
文献类型：
--
作者：
Azuma MM;Balani P;Boisvert H;Gil M;Egashira K;Yamaguchi T;Hasturk H;Duncan M;Kawai T;Movila A
神经酰胺酶是一组通过裂解脂肪酸以形成抗炎鞘氨醇脂质来降解促炎性神经酰胺的酶。到目前为止，已经描述了酸性、中性和碱性神经酰胺酶同工酶。然而，神经酰胺酶亚型的表达模式以及它们在牙周病发病机制中的作用仍然未知。在本研究中，通过实时PCR和免疫组织化学定量了牙周炎患者和健康受试者的牙龈样品中以及EpiGingival™-3D培养物和OBA-9牙龈上皮细胞中神经酰胺酶同种型的表达模式，两者均在存在或不存在活牙龈卟啉单胞菌（ATCC 33277菌株）的情况下刺激。牙周病患者牙龈组织中酸性神经酰胺酶的表达水平显著低于健康人。此外，EpiGingival™ 3D培养物和OBA-9细胞中的酸性神经酰胺酶表达通过体外牙龈卟啉单胞菌刺激而受到抑制。中性或碱性神经酰胺酶在牙龈样本或细胞培养物中均未检测到显著波动。接下来，为了阐明酸性神经酰胺酶在体外牙龈卟啉单胞菌诱导的炎症中的作用，用表达人酸性神经酰胺酶（Ad-ASAH 1）基因的腺病毒载体或对照腺病毒载体（Ad-对照）转导OBA-9细胞。在牙龈卟啉单胞菌的刺激下，ASAH 1过表达的OBA-9细胞与Ad对照载体转导的OBA-9细胞相比，caspase-3的mRNA表达以及Annexin V阳性细胞的百分比显著降低。此外，响应于牙龈卟啉单胞菌的刺激，ASAH 1过表达的OBA-9细胞产生的TNF-α、IL-6和IL 1 β促炎细胞因子比用Ad对照载体转导的OBA-9细胞中观察到的少。总的来说，我们的数据显示了新发现的抗炎和抗凋亡作用的酸性神经酰胺酶在宿主细胞暴露于牙周细菌，和衰减的宿主保护性酸性神经酰胺酶在牙周病变的表达。
Ceramidases are a group of enzymes that degrade pro-inflammatory ceramide by cleaving a fatty acid to form anti-inflammatory sphingosine lipid. Thus far, acid, neutral and alkaline ceramidase isozymes have been described. However, the expression patterns of ceramidase isoforms as well as their role in periodontal disease pathogenesis remain unknown. In this study, expression patterns of ceramidase isoforms were quantified by real-time PCR and immunohistochemistry in gingival samples of patients with periodontitis and healthy subjects, as well as in EpiGingival™-3D culture and OBA-9 gingival epithelial cells both of which were stimulated with or without the presence of live Porphyromonas gingivalis (ATCC 33277 strain). A significantly lower level of acid ceramidase expression was detected in gingival tissues from periodontal patients compared to those from healthy subjects. In addition, acid-ceramidase expression in EpiGingival™ 3D culture and OBA-9 cells was suppressed by stimulation with P. gingivalis in vitro. No significant fluctuation was detected for neutral or alkaline ceramidases in either gingival samples or cell cultures. Next, to elucidate the role of acid ceramidase in P. gingivalis-induced inflammation in vitro, OBA-9 cells were transduced with adenoviral vector expressing the human acid ceramidase (Ad-ASAH1) gene or control adenoviral vector (Ad-control). In response to stimulation with P. gingivalis, ASAH1-over-expressing OBA-9 cells showed significantly lower mRNA expressions of caspase-3 as well as the percentage of Annexin V-positive cells, when compared with OBA-9 cells transduced with Ad-control vector. Furthermore, in response to stimulation with P. gingivalis, ASAH1-over-expressing OBA-9 cells produced less TNF-α, IL-6, and IL1β pro-inflammatory cytokines than observed in OBA-9 cells transduced with Ad-control vector. Collectively, our data show the novel discovery of anti-inflammatory and anti-apoptotic effects of acid ceramidase in host cells exposed to periodontal bacteria, and the attenuation of the expression of host-protective acid ceramidase in periodontal lesions.
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影响因子：
4.8
作者：
Eliyahu, Efrat;Park, Jae-Ho;Schuchman, Edward H.
通讯作者：
Schuchman, Edward H.
DOI：
10.1006/bbrc.2000.3370
发表时间：
2000-09-07
影响因子：
3.1
作者：
Romiti, E;Meacci, E;Bruni, P
通讯作者：
Bruni, P
影响因子：
6.5
作者：
Houben, Evi;Holleran, Walter M.;Uchida, Yoshikazu
通讯作者：
Uchida, Yoshikazu
影响因子：
9
作者：
Wang K;Xu R;Snider AJ;Schrandt J;Li Y;Bialkowska AB;Li M;Zhou J;Hannun YA;Obeid LM;Yang VW;Mao C
通讯作者：
Mao C
碱性神经酰胺酶 2 是 p53 的新型直接靶标，通过 ROS 生成诱导自噬和细胞凋亡
影响因子：
4.6
作者：
Wang Y;Zhang C;Jin Y;Wang;He Q;Liu Z;Ai Q;Lei Y;Li Y;Song F;Bu Y
通讯作者：
Bu Y