In vitro activities of nine antifungal agents against rare pathogenic fungi.
In vitro activities of nine antifungal agents against rare pathogenic fungi.
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DOI：
10.1099/jmm.0.001083
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发表时间：
2019-11
影响因子：
3
通讯作者：
Lingyue Sun;Z. Wan;Ruoyu Li;Jin Yu
中科院分区：
文献类型：
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作者：
Lingyue Sun;Z. Wan;Ruoyu Li;Jin Yu
目的.评价9种抗真菌药（阿替西霉素B、氟康唑、伏立康唑、伊曲康唑、泊沙康唑、卡泊芬净、米卡芬净、特比萘芬和5-氟胞嘧啶）对93株罕见致病真菌的体外活性，以及联合用药对几种多重耐药真菌的联合作用。本研究采用美国临床和实验室标准研究所的微量肉汤稀释法M38-A3和M27-A4以及棋盘法。氟康唑在霉菌（包括小偃麦、外瓶霉和酵母）中的MIC较低。在构巢曲霉、镰刀菌、长枝木霉、小黑麦、柏氏小克银汉霉、褐色小克银汉霉、冠状耳霉、外瓶霉、奇异赭曲霉和巴西鼻枝霉中发现了阿替霉素B>2 µg ml-1的MIC。多药耐药性在微囊藻属中观察到，多生长孢霉和阴险腐霉。结论。本研究阐明了几种常见致病真菌的体外药物敏感性，为临床治疗真菌感染提供了依据。
Purpose . To assess in vitro activities of nine antifungal agents (amphotericin B, fluconazole, voriconazole, itraconazole, posaconazole, caspofungin, micafungin, terbinafine and 5-flucytosine) against 93 strains of rare pathogenic fungi and the combined effects of drug combinations against several multidrug-resistant fungi.Methodology . The broth microdilution method M38-A3 and M27-A4 from the Clinical and Laboratory Standards Institute and the checkerboard method were performed in this study.Results . Low MICs for fluconazole were observed in moulds including Tritirachium oryzae, Exophiala attenuata and yeasts. MICs for amphotericin B>2 µg ml-1 were found among Aspergillus nidulans, Fusarium napiforme, Trichoderma longibrachiatum, Tritirachium oryzae, Cunninghamella bertholletiae, Cunninghamella phaeospora, Conidiobolus coronatus, Exophiala attenuata, Ochroconis mirabilis and Rhinocladiella basitona. Multidrug resistance was observed in Microascus spp., Lomentospora prolificans and Pythium insidiosum.Conclusion . Our study illustrated in vitro drug susceptibilities of some rare pathogenic fungi, which provide data to guide clinical treatment of fungal infections.