Sensitivity of indirect immunofluorescence, substrate specificity, and immunoblotting in the diagnosis of pemphigus
Sensitivity of indirect immunofluorescence, substrate specificity, and immunoblotting in the diagnosis of pemphigus
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DOI：
10.1016/s0190-9622(97)80127-2
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发表时间：
1997-08-01
影响因子：
13.8
通讯作者：
Bystryn, JC
中科院分区：
文献类型：
--
作者：
Jiao, D;Bystryn, JC
背景：有几种方法可用于检测天疱疮抗体。方法：对41例寻常型天疱疮（PV）患者和22例落叶型天疱疮（PF）患者的52份血清同时进行检测，比较两种方法检测天疱疮抗体的敏感性。结果：IIF检测PV和PF患者的天疱疮抗体具有相同的灵敏度（即，阳性率分别为87%和86%。相比之下，IB测定在PF中的灵敏度远低于PV（即，分别在45%和83%的血清中呈阳性）。PV中的抗体通常对猴食管反应更强烈，而PF中的抗体对豚鼠食管反应更强烈。所有患者的细胞间抗体，反应更强烈地对猴子比豚鼠食管PV，而所有的细胞间抗体，反应更强烈地对豚鼠比猴子食管PF。结论：IIF是一个更敏感的检测方法比IB与PE相关的抗体的底物特异性的抗体提供了一个简单的方法来区分PV和PF。
Background: Several assays are available to detect pemphigus antibodies. The most commonly used are indirect immunofluorescence (IIF) and immunoblotting (IB).Objective: Our purpose was to compare the sensitivity of these assays in detecting pemphigus antibodies.Methods: Fifty-two sera from 41 patients with pemphigus vulgaris (PV) and 22 sera from is patients with pemphigus foliaceus (PF) were tested concurrently for the presence of pemphigus antibodies. The IIF studies were conducted with two different substrate: monkey and guinea pig esophagus.Results: Pemphigus antibodies were detected with equal sensitivity by IIF in patients with PV and PF (i.e., positive in 87% and 86% of sera, respectively). By contrast, IB assay was much less sensitive in PF than in PV (i.e., positive in 45% vs 83% of sera, respectively). The antibodies in PV generally reacted more strongly against monkey esophagus, whereas those in PF reacted more strongly against guinea pig esophagus. All patients with intercellular antibodies that reacted more strongly against monkey than guinea pig esophagus had PV, whereas all those with intercellular antibodies that reacted more strongly against guinea pig than monkey esophagus had PF.Conclusion: IIF is a more sensitive assay than IB for detecting antibodies associated with PE The substrate specificity of the antibodies provides a simple means to distinguish between PV and PF.