CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores.
CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores.
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CW EPR 和 DEER 方法确定 BCL-2 家族蛋白质结构和相互作用：定点自旋标记在 BAK 凋亡孔中的应用。
DOI：
10.1007/978-1-4939-8861-7_18
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Oh,KyoungJoon
中科院分区：
文献类型：
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作者：
Mandal,Tirtha;Hustedt,EricJ;Song,Likai;Oh,KyoungJoon
连续波(CW)和脉冲电子顺磁共振(EPR)方法能够测量包括蛋白质在内的生物聚合物中自旋标记残基之间的距离，提供结构信息。在这里，我们描述了连续波EPR去卷积/卷积方法和四脉冲双电子-电子共振(DER)距离测定方法，这两种方法被用来解释形成在脂质双层中的BAK凋亡孔的组织。
The continuous wave (CW) and pulse electron paramagnetic resonance (EPR) methods enable the measurement of distances between spin-labeled residues in biopolymers including proteins, providing structural information. Here we describe the CW EPR deconvolution/convolution method and the four-pulse double electron–electron resonance (DEER) approach for distance determination, which were applied to elucidate the organization of the BAK apoptotic pores formed in the lipid bilayers.