Radiation inactivation studies of the renal brush-border membrane phlorizin-binding protein.
Radiation inactivation studies of the renal brush-border membrane phlorizin-binding protein.
复制标题
肾刷状缘膜根皮苷结合蛋白的辐射灭活研究。
DOI：
10.1016/s0021-9258(18)33894-8
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
E. Kempner
中科院分区：
文献类型：
--
作者：
R. Turner;E. Kempner
用高能电子束辐照肾刷状膜囊泡。整体膜蛋白、碱性磷酸酶和5′-核苷酸酶的活性随辐射剂量呈单指数损失，分别表明靶大小为67,000道尔顿和58,000道尔顿。钠依赖性的根连素与刷缘膜d -葡萄糖转运体结合的失活更为复杂。即使是最小剂量的辐射，也会丢失一半的根霉素结合位点，这表明根霉素结合蛋白亚群具有较大的功能单位（大于4 X 10(6)道尔顿）。其余的位点表现为110,000 +/- 8,000道尔顿的单一辐射靶标，并保留了通常与根霉素结合葡萄糖转运体相关的动力学特征。因此，这些数据与刷边膜d -葡萄糖转运蛋白的根霉素结合部分的分子量为110,000的分配是一致的。
Renal brush-border membrane vesicles were irradiated in the frozen state with a high energy electron beam. The integral membrane proteins, alkaline phosphatase and 5'-nucleotidase, each showed a single exponential loss of activity with radiation dose, indicating target sizes of 67,000 and 58,000 daltons, respectively. Inactivation of sodium-dependent phlorizin binding to the brush-border membrane D-glucose transporter was more complex. One-half of the phlorizin binding sites were lost after even the smallest doses of radiation suggestive of large functional units (greater than 4 X 10(6) daltons) for a subpopulation of phlorizin binding proteins. The remaining sites behaved as a single radiation target of 110,000 +/- 8,000 daltons and retained the kinetic characteristics commonly associated with phlorizin binding to the glucose transporter. Thus, the data are consistent with the assignment of a molecular weight of 110,000 to the phlorizin binding moiety of the brush-border membrane D-glucose transport protein.