Intratumoral Wnt1 expression affects survivin gene expression in non-small cell lung cancer
Intratumoral Wnt1 expression affects survivin gene expression in non-small cell lung cancer
复制标题
DOI：
10.3892/ijo_00000718
复制
发表时间：
2010-09-01
影响因子：
5.2
通讯作者：
Yokomise, Hiroyasu
中科院分区：
文献类型：
--
作者：
Nakashima, Nariyasu;Huang, Cheng-Long;Yokomise, Hiroyasu
Survivin是凋亡抑制蛋白家族的一员，影响肿瘤的发生。最近，据报道生存素是经典Wnt通路的靶点，其激活多种肿瘤相关靶基因的转录。研究了122例非小细胞肺癌（NSCLC），以评估生存素基因表达与Wnt 1（经典Wnt通路的新成员）和Wnt 5a（非经典Wnt通路的新成员）表达的关系。半定量RT-PCR检测Survivin基因的表达。免疫组化法检测pan-survivin、Wnt 1和Wn 5a蛋白的表达。同时检测细胞凋亡指数和Ki-67增殖指数。64例（52.5%）为高生存素肿瘤，65例为高Wnt 1肿瘤，67例（54.9%）为高Wnt 5a肿瘤。Survivin基因标准化表达与凋亡指数呈显著正相关（P
Survivin, a member of the inhibitor of apoptosis protein family, affects tumorigenesis. Recently, survivin is reported to be a target of the canonical Wnt pathway, which activates the transcription of various tumor-associated target genes. One hundred and twenty-two non-small cell lung cancers (NSCLCs) were investigated to evaluate survivin gene expression in relation to the expression of Wnt1 (a novel member of the canonical Wnt pathway) and Wnt5a (a novel member of the non-canonical Wnt pathway). The survivin gene expression was evaluated by semi-quantitative RT-PCR. The protein expression of pan-survivin, Wnt1, and Wn5a were investigated by immunohistochemistry. The apoptotic index and the Ki-67 proliferation index were also evaluated. Sixty-four tumors (52.5%) were survivin-high tumors, 65 tumors were Wnt1-high tumors, and 67 tumors (54.9%) were Wnt5a-high tumors. The standardized survivin gene expression significantly correlated with the apoptotic index (P