Solvent Effects on the Ultrastructure and Chemical Composition of Cuticular Wax and Its Potential Bioactive Role Against Alternaria alternate in Pingguoli Pear
Solvent Effects on the Ultrastructure and Chemical Composition of Cuticular Wax and Its Potential Bioactive Role Against Alternaria alternate in Pingguoli Pear
复制标题
溶剂对苹果梨表皮蜡超微结构和化学成分的影响及其抗链格孢的潜在生物活性
DOI：
10.1016/s2095-3119(13)60374-3
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发表时间：
2014-01-01
影响因子：
4.8
通讯作者：
Wang Yi
中科院分区：
文献类型：
--
作者：
Chen Song-jiang;Li Yong-cai;Wang Yi
研究了不同极性溶剂对苹果梨表皮蜡的超微结构、化学组成的影响及其对互隔交链孢菌的生物活性作用。结果表明，氯仿提取的蜡含量最高，其蜡含量达322.2 μ g cm（-2）。薄荷醇提取物中长链脂肪酸占优势，乙醚、氯仿和正己烷提取的蜡中正构烷烃占优势。苹果梨果实表面覆盖着一层光滑无定形的蜡质层，内含细小、分散的晶体。离体条件下，不同溶剂去除后的重结晶蜡的形态与完整果实表面的形态不同。用各种溶剂去除表皮蜡显著提高了A。alternata感染，除了用甲醇除去蜡。甲醇和氯仿的溶剂提取物能促进孢子萌发和菌丝生长。alternata，但乙醚和正己烷提取物显示出抗真菌活性。
Effects of different polarity solvents on the ultrastructure and chemical composition of cuticular wax in Pingguoli pear as well as their bioactive role against Alternaria alternate were studied and the results showed that the highest wax content was extracted with chloroform, and its wax content was up to 322.2 mu g cm(-2). Long-chain fatty acids predominated in menthol extracts and n-alkanes were predominant in wax extracted with ether, chloroform and n-hexane. Pingguoli pear fruit surface was covered by a smooth and amorphous wax layer with small, scattered crystal. The morphology of recrystallized wax in vitro after removal with different solvents was not similar to that of the intact fruit surface. Removal of cuticular wax with various solvents significantly enhanced A. alternata infection, except for wax removed by methanol. The solvent extracts of methanol and chloroform stimulated the spore germination and mycelium growth of A. alternata, but the ether and n-hexane extracts showed antifungal activity.