Human cytomegalovirus induces interleukin-8 production by a human monocytic cell line, THP-1, through acting concurrently on AP-1- and NF-kappa B-binding sites of the interleukin-8 gene
Human cytomegalovirus induces interleukin-8 production by a human monocytic cell line, THP-1, through acting concurrently on AP-1- and NF-kappa B-binding sites of the interleukin-8 gene
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DOI：
10.1128/jvi.71.7.5692-5695.1997
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发表时间：
1997-07-01
影响因子：
5.4
通讯作者：
Matsushima, K
中科院分区：
文献类型：
--
作者：
Murayama, T;Ohara, Y;Matsushima, K
巨细胞病毒（Cytomegalovirus，CMV）感染诱导人单核细胞系THP-1细胞IL-8基因的转录，导致IL-8的分泌。IL-X基因的功能分析表明，AP-1和NF-κ E因子结合元件都参与了对CMV的应答。此外，电泳迁移率变动分析表明，CMV诱导NF-κ B和AP-1复合物的形成。这些结果表明，CMV激活这些转录因子，导致IL-8基因表达。
Cytomegalovirus (CMV) infection induced interleukin-8 (IL-S) gene transcription in a human monocytic cell line, THP-1 cells, leading to IL-S secretion, The functional analysis of the IL-X gene revealed that both AP-1-and NF-kappa E factor-binding elements were involved in conferring the responsiveness to CMV. Moreover, electrophoretic mobility shift assays demonstrated that CMV induced the formation of NF-kappa B and AP-1 complexes. These results suggest that CMV activates these transcriptional factors, resulting in IL-8 gene expression.