An HPLC method to determine tryptophan and kynurenine in serum simultaneously.
An HPLC method to determine tryptophan and kynurenine in serum simultaneously.
复制标题
高效液相色谱法同时测定血清中色氨酸和犬尿氨酸。
DOI：
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发表时间：
1999
影响因子：
--
通讯作者：
Dietmar Fuchs
中科院分区：
文献类型：
--
作者：
B. Widner;E. R. Werner;H. Schennach;Dietmar Fuchs
建立了同时测定血清色氨酸和犬尿氨酸的方法。色氨酸在干扰素-γ诱导的吲哚胺2，3-双加氧酶的作用下被转化为犬尿氨酸。由于干扰素-γ是一种Th1细胞衍生的细胞因子，当细胞免疫系统被激活时，例如在病毒感染或自身免疫性疾病中，可以发现通过犬尿氨酸途径增加的色氨酸降解率。因此，犬尿氨酸/色氨酸的比值提供了评估体内干扰素-γ活性的可能性，并进一步反映了免疫激活的程度。高效液相色谱法需要100微升的血清。蛋白质是用三氯乙酸除去的。使用基于白蛋白的外部校准器，并参考内标3-硝基-L酪氨酸进行分析。通过360 nm的紫外吸收检测犬尿氨酸和硝基酪氨酸，通过在285 nm消光和365 nm发射的天然荧光检测色氨酸。测定了49例健康献血员血清中犬尿氨酸和色氨酸的代表性正常值。
A method was established to measure tryptophan and kynurenine in serum simultaneously. Tryptophan is converted to kynurenine by the action of the enzyme indoleamine 2,3-dioxygenase induced by interferon-gamma (IFN-gamma). Since IFN-gamma is a Th1-cell derived cytokine, an increased tryptophan degradation rate via the kynurenine pathway can be found when the cellular immune system is activated as it is, e.g., in viral infections or in autoimmune diseases. Thus, the ratio kynurenine per tryptophan provides a possibility to estimate IFN-gamma activity in vivo and furthermore reflects the degree of immune activation. The HPLC method requires 100 microL serum. Protein is removed by trichloroacetic acid. An external albumin-based calibrator is applied, and analysis is referred to an internal standard, 3-nitro-L-tyrosine. Kynurenine and nitrotyrosine are detected via UV absorbance at 360 nm wavelength, and tryptophan is detected via its natural fluorescence at 285 nm extinction and 365 nm emission. Representative normal values of kynurenine and tryptophan were measured in the sera of 49 healthy blood donors.
影响因子：
15.9
作者：
HADDAD, IY;PATAKI, G;MATALON, S
通讯作者：
MATALON, S