Micro‐XRD and nanoindentation investigation of bioceramics for dental pulp therapy
Micro‐XRD and nanoindentation investigation of bioceramics for dental pulp therapy
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用于牙髓治疗的生物陶瓷的显微 XRD 和纳米压痕研究
DOI：
10.1002/mds3.10027
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Mizoguchi I
中科院分区：
文献类型：
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作者：
Alapati SB;Iijima M;Brantley WA;Ito S;Muguruma T;Saito T;Mizoguchi I
用于牙髓治疗的治疗性生物陶瓷是一个活跃的研究领域。本研究旨在探讨两种常用生物陶瓷在使用后的阶段及牙本质矿化的深度。用ProRoot白色矿物三氧化物聚集体（MTA）（Dentsply塔尔萨牙科公司）、EndoSequence牙根修复材料（Desseler USA）填充非龋性第三磨牙的牙洞，并用氢氧化钙（Dycal，Dentsply Caulk）作为对照。将样本浸入模拟体液（SBF）中3个月，并在放置后2分钟和随后的7个时间间隔对生物陶瓷进行微X射线衍射（Micro-XRD）分析。在SBF中三个月后，对试样进行纳米压痕测试。Micro-XRD显示在两种生物陶瓷中均存在硅酸二钙、硅酸三钙、铝酸三钙和硫酸钙。在ProRoot白色MTA和EndoSequence牙根修复材料中还分别检测到氧化铋和氧化锆的乳浊剂。没有证据表明这些生物陶瓷在凝固过程中形成硅酸盐凝胶基质。纳米压痕测试显示，在距离界面<17 µm处，Dycal填充的样本中牙本质的纳米硬度低于EndoSequence和ProRoot白色MTA，而在距离界面21-97 µm处，任何材料填充的牙本质的纳米硬度均无显著差异。
Therapeutic bioceramics for dental pulp treatment are an area of active research. This study investigated the phases in two commonly used bioceramics and depth of dentin remineralization after application of these bioceramics. Cavities in non‐carious third molars were filled with ProRoot White mineral trioxide aggregate (MTA) (Dentsply Tulsa Dental), EndoSequence Root Repair Material (Brasseler USA) and with calcium hydroxide (Dycal, Dentsply Caulk) serving as a control. Specimens were immersed in simulated body fluid (SBF) for 3 months, and Micro‐X‐ray diffraction (Micro‐XRD) analyses were performed on the bioceramics two minutes after placement and at seven subsequent intervals. After three months in SBF, specimens were subjected to nanoindentation testing. Micro‐XRD revealed the presence of dicalcium silicate, tricalcium silicate, tricalcium aluminate and calcium sulphate in both bioceramics. Opacifying agents bismuth oxide and zirconia were also detected in ProRoot White MTA and EndoSequence Root Repair Material, respectively. There was no evidence for the silicate gel matrix that forms during setting of these bioceramics. Nanoindentation testing revealed that at <17 µm from the interface, the nanohardness of dentin in specimens filled with Dycal was lower than for EndoSequence and ProRoot White MTA, whereas at 21–97 µm from the interface, there was no significant difference in nanohardness of dentin filled with any material.