Differential expression of crystallin genes during development of the rat eye lens.
Differential expression of crystallin genes during development of the rat eye lens.
复制标题
大鼠晶状体发育过程中晶状体蛋白基因的差异表达。
DOI：
10.1016/0012-1606(87)90249-1
复制
发表时间：
1987
影响因子：
2.7
通讯作者：
John G. G. Schoenmakers
中科院分区：
文献类型：
--
作者：
Rob W. van Leen;K. Roozendaal;N. Lubsen;John G. G. Schoenmakers
使用 Northern 印迹和斑点印迹分析，在大鼠晶状体发育过程中测量 αA-、βB1- 和总 γ-晶状体蛋白 mRNA 的浓度。胎儿生长 14 天后，观察到所有三种 mRNA 浓度急剧增加。出生后，αA-晶状体蛋白转录物浓度一直保持较高水平，直至6月龄，γ-晶状体蛋白转录物浓度逐渐下降，而βB1-晶状体蛋白转录物浓度急剧下降。通过使用引物延伸和 S1 核酸酶作图测量六个 γ-晶状体蛋白基因中每一个的转录物的相对量来确定 γ-晶状体蛋白 mRNA 库的组成。所有六个基因的转录本直到出生后第三个月才被发现。此后，γ1-1、γ3-1 和γ4-1 晶状体蛋白基因的转录本不再可检测到。随后，在 8 个月大时，γ2-1 和 γ2-2 基因的转录本也消失，仅留下 γ1-2 晶状体蛋白基因的转录本。因此，六种不同的 γ-晶状体蛋白 mRNA 的浓度受到不同的调节。
The concentrations of αA-, βB1-, and total γ-crystallin mRNAs were measured during development of the rat eye lens, using Northern blot and dot blot analysis. After 14 days of fetal growth a sharp increase in the concentration of all three mRNA species was observed. After birth, the concentration of αA-crystallin transcripts remains high until 6 months of age, the concentration of γ-crystallin transcripts decreases gradually, while the concentration of βB1-crystallin transcripts decreases sharply. The composition of the γ-crystallin mRNA pool was determined by measuring the relative amount of the transcripts of each of the six γ-crystallin genes using primer extension and S1-nuclease mapping. The transcripts of all six genes are found until the third month after birth. Thereafter the transcripts of the γ1-1, the γ3-1, and γ4-1 crystallin genes are no longer detectable. Later on the transcripts of the γ2-1 and γ2-2 genes also disappear leaving only the transcripts of the γ1-2 crystallin gene at the age of 8 months. The concentration of the six different γ-crystallin mRNAs is thus regulated differentially.
由特定寡核苷酸引物启动锥虫可变抗原 mRNA 的逆转录。
DOI：
10.1073/pnas.80.6.1536
发表时间：
1983
影响因子：
11.1
作者：
Merritt,SC;Tschudi,C;Konigsberg,WH;Richards,FF
通讯作者：
Richards,FF