Eavesdropping wires: Recording activity in axons using genetically encoded calcium indicators.
Eavesdropping wires: Recording activity in axons using genetically encoded calcium indicators.
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DOI：
10.1016/j.jneumeth.2021.109251
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发表时间：
2021-08-01
影响因子：
3
通讯作者：
Petreanu L
中科院分区：
文献类型：
--
作者：
Broussard GJ;Petreanu L
神经元通过广泛的轴突将电信号传送到大脑的远端区域。基因编码的钙传感器允许直接观察轴突终末的动作电位活动，提供对神经投射的组织和功能的独特见解。在这里，我们考虑从轴突记录中可以收集到什么信息，从从多个细胞轴突投影提取的总和活动到单个牛顿。特别是，我们讨论了最近发展的不同的多光子和纤维光度测量方法在记录啮齿类动物轴突中神经活动的应用。我们定义了与轴索室成像方法相关的实验困难，并强调了解决这些问题的最新方法学进展。最后，我们反思了如何将新技术与轴突钙成像结合使用来解决当前神经生物学中的问题。
Neurons broadcast electrical signals to distal brain regions through extensive axonal arbors. Genetically encoded calcium sensors permit the direct observation of action potential activity at axonal terminals, providing unique insights on the organization and function of neural projections. Here, we consider what information can be gleaned from axonal recordings made at scales ranging from the summed activity extracted from multi-cell axon projections to single boutons. In particular, we discuss the application of different recently developed multi photon and fiber photometry methods for recording neural activity in axons of rodents. We define experimental difficulties associated with imaging approaches in the axonal compartment and highlight the latest methodological advances for addressing these issues. Finally, we reflect on ways in which new technologies can be used in conjunction with axon calcium imaging to address current questions in neurobiology.
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