Different postharvest conditions modulate ripening and ethylene biosynthetic and signal transduction pathways in Stony Hard peaches
Different postharvest conditions modulate ripening and ethylene biosynthetic and signal transduction pathways in Stony Hard peaches
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DOI：
10.1016/j.postharvbio.2007.09.023
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发表时间：
2008-04-01
影响因子：
7
通讯作者：
Tonutti, Pietro
中科院分区：
文献类型：
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作者：
Begheldo, Maura;Manganaris, George A.;Tonutti, Pietro
石硬（SH）桃的特征在于，在成熟时，由于P-p-ACS 1的表达减少，果肉硬度的维持和乙烯产生的缺乏。在一项试验中，比较融化的肉（MF，cv。“Summer Rich”）和SH（“IFF 331”选择）果实在两种不同的采后温度（10和20摄氏度）下贮藏时，在SH桃中观察到意想不到的行为，当在10摄氏度下贮藏时，显示乙烯产生增加和果肉硬度降低，10摄氏度是一种在延迟MF果实成熟方面基本无效的温度制度。这似乎是P-p-ACS 1转录诱导的结果，使得该基因型对于研究桃中的温度胁迫生理和乙烯相关的成熟过程特别感兴趣。细胞壁代谢相关基因的比较表达分析指出，存在一个负（P-P-EG 4），正（Pp-endoPG）或没有（PL家族的一个成员）与乙烯在成熟的关系。结果清楚地表明，硬度下降的最后阶段（融化）只发生在产生乙烯的果实中，并与P-p-endoPG转录本积累有关。使用QRT-PCR评估参与乙烯生物合成和信号传导途径的基因的表达。Pp-ACO 1在10 ℃的SH中出现诱导，但在20 ℃时没有。Pp-CTR 1和Pp-E1 N2样基因表达的瞬时增加仅在产生乙烯的样品中的成熟的早期阶段被检测到，这表明在桃果实成熟过程中乙烯和其转导途径的元件之间可能存在因果关系。(c)2007 Elsevier B. V.保留所有权利。
Stony hard (SH) peaches are characterized, at ripening, by the maintenance of flesh firmness and the lack of ethylene production due to a reduced expression of P-p-ACS1. In a trial comparing melting flesh (MF, cv. 'Summer Rich') and SH ('IFF331' selection) fruit at two different postharvest temperatures (10 and 20 degrees C), unexpected behaviour was observed in SH peaches that displayed an increase in ethylene production and a decrease in flesh firmness when stored at 10 degrees C, a temperature regime basically ineffective in delaying ripening in MF fruit. This appeared to be the result of an induction of P-p-ACS1 transcription, making this genotype of particular interest for studying temperature stress physiology and ethylene-related ripening processes in peaches. Comparative expression analyses of genes involved in cell wall metabolism pointed out the presence of a negative (P-p-EG4), positive (Pp-endoPG) or no (one member of the PL family) relationship with ethylene at ripening. Results clearly showed that the last stage of firmness decrease (melting) only occurs in fruit producing ethylene and is associated with P-p-endoPG transcript accumulation. The expression of genes involved in ethylene biosynthesis and signalling pathways was evaluated using QRT-PCR. Pp-ACO1 appeared to be induced in SH kept at 10 degrees C but not at 20 degrees C. Transient increases in Pp-CTR1 and Pp-E1N2like gene expression have only been detected at the early stages of ripening in samples producing ethylene, indicating that a causal relationship might exist between ethylene and elements of its transduction pathway during peach fruit ripening. (c) 2007 Elsevier B.V. All rights reserved.